Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)

To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA cl...

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Autores principales:	Tang,Yue Yi, Wang,Chuan Tang, Yang,Guan Pin, Feng,Tong, Gao,Hua Yuan, Wang,Xiu Zhen, Chi,Xiao Yuan, Xu,Ya Long, Wu,Qi, Chen,Dian Xu
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	chilling response peanut real-time quantitative PCR suppression subtractive hybridization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
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spelling	oai:scielo:S0717-345820110005000052012-04-17Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)Tang,Yue YiWang,Chuan TangYang,Guan PinFeng,TongGao,Hua YuanWang,Xiu ZhenChi,Xiao YuanXu,Ya LongWu,QiChen,Dian Xu chilling response peanut real-time quantitative PCR suppression subtractive hybridization To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.5 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	chilling response peanut real-time quantitative PCR suppression subtractive hybridization
spellingShingle	chilling response peanut real-time quantitative PCR suppression subtractive hybridization Tang,Yue Yi Wang,Chuan Tang Yang,Guan Pin Feng,Tong Gao,Hua Yuan Wang,Xiu Zhen Chi,Xiao Yuan Xu,Ya Long Wu,Qi Chen,Dian Xu Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
description	To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
author	Tang,Yue Yi Wang,Chuan Tang Yang,Guan Pin Feng,Tong Gao,Hua Yuan Wang,Xiu Zhen Chi,Xiao Yuan Xu,Ya Long Wu,Qi Chen,Dian Xu
author_facet	Tang,Yue Yi Wang,Chuan Tang Yang,Guan Pin Feng,Tong Gao,Hua Yuan Wang,Xiu Zhen Chi,Xiao Yuan Xu,Ya Long Wu,Qi Chen,Dian Xu
author_sort	Tang,Yue Yi
title	Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
title_short	Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
title_full	Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
title_fullStr	Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
title_full_unstemmed	Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
title_sort	identification of chilling-responsive transcripts in peanut (arachis hypogaea l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
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Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)

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