Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14

Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture c...

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Autores principales:	Moura Leal,Maria de Lourdes, da Silva Gomes Pereira,Denise, Jessouroun,Ellen, Peixoto Gimenes Couto,Maria Antonieta, Pereira Jr,Nei
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	polysaccharide capsular vaccine Streptococcus pneumoniae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006
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spelling	oai:scielo:S0717-345820110005000062012-04-17Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14Moura Leal,Maria de Lourdesda Silva Gomes Pereira,DeniseJessouroun,EllenPeixoto Gimenes Couto,Maria AntonietaPereira Jr,Nei polysaccharide capsular vaccine Streptococcus pneumoniae Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an inhibitory effect on cell growth in concentrations ranging from 4 to 5 g/L. An increase of 30% in polysaccharide productivity was observed using glucose pulses with 5.5 hrs of growth, resulting in a maximum polysaccharide concentration of 185.2 mg/L. Our data suggest the possibility of using a medium of non-animal origin and employing pH control for the cultivation of pneumococcus to produce a polysaccharide vaccine.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.5 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	polysaccharide capsular vaccine Streptococcus pneumoniae
spellingShingle	polysaccharide capsular vaccine Streptococcus pneumoniae Moura Leal,Maria de Lourdes da Silva Gomes Pereira,Denise Jessouroun,Ellen Peixoto Gimenes Couto,Maria Antonieta Pereira Jr,Nei Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
description	Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an inhibitory effect on cell growth in concentrations ranging from 4 to 5 g/L. An increase of 30% in polysaccharide productivity was observed using glucose pulses with 5.5 hrs of growth, resulting in a maximum polysaccharide concentration of 185.2 mg/L. Our data suggest the possibility of using a medium of non-animal origin and employing pH control for the cultivation of pneumococcus to produce a polysaccharide vaccine.
author	Moura Leal,Maria de Lourdes da Silva Gomes Pereira,Denise Jessouroun,Ellen Peixoto Gimenes Couto,Maria Antonieta Pereira Jr,Nei
author_facet	Moura Leal,Maria de Lourdes da Silva Gomes Pereira,Denise Jessouroun,Ellen Peixoto Gimenes Couto,Maria Antonieta Pereira Jr,Nei
author_sort	Moura Leal,Maria de Lourdes
title	Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
title_short	Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
title_full	Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
title_fullStr	Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
title_full_unstemmed	Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14
title_sort	investigation of cultivation conditions for capsular polysaccharide production by streptococcus pneumoniae serotype 14
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006
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Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14

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