Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis

Anaerobic sludge from palm oil mill effluent (POME) treatment plant was used as a source of inocula for the conversion of POME into hydrogen. Optimization of temperature and initial pH for biohydrogen production from POME was investigated by response surface methodology. Temperature of 60ºC and init...

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Autores principales:	O-Thong,Sompong, Mamimin,Chonticha, Prasertsan,Poonsuk
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	biohydrogen long-term evaluation palm oil mill effluent response surface methodology thermophilic condition
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500009
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spelling	oai:scielo:S0717-345820110005000092012-04-17Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysisO-Thong,SompongMamimin,ChontichaPrasertsan,Poonsuk biohydrogen long-term evaluation palm oil mill effluent response surface methodology thermophilic condition Anaerobic sludge from palm oil mill effluent (POME) treatment plant was used as a source of inocula for the conversion of POME into hydrogen. Optimization of temperature and initial pH for biohydrogen production from POME was investigated by response surface methodology. Temperature of 60ºC and initial pHof 5.5 was optimized for anaerobic microflora which gave a maximum hydrogen production of 4820 ml H2/l-POME corresponding to hydrogen yield of 243 ml H2/g-sugar. Total sugar consumption and chemical oxygen demand (COD) removal efficiency were 98.7% and 46%, respectively. Long-term hydrogen production in continuous reactor at HRT of 2 days, 1 day and 12 hrs were 4850 ± 90, 4660 ± 99 and 2590 ± 120 ml H2/l-POME, respectively. Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and continuous reactors were phylogenetically related to the Thermoanaerobacterium thermosaccharolyticum. Batch reactor showed more diversity of microorganisms than continuous reactor. Microbial community structure of batch reactor was comprised of T. thermosaccharolyticum, T. bryantii, Thermoanaerobacterium sp., Clostridium thermopalmarium and Clostridium NS5-4, while continuous reactor was comprised of T. thermosaccharolyticum, T. bryantii and Thermoanaerobacterium sp. POME is good substrate for biohydrogen production under thermophilic condition with Thermoanaerobacterium species play an important role in hydrogen fermentation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.5 20112011-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biohydrogen long-term evaluation palm oil mill effluent response surface methodology thermophilic condition
spellingShingle	biohydrogen long-term evaluation palm oil mill effluent response surface methodology thermophilic condition O-Thong,Sompong Mamimin,Chonticha Prasertsan,Poonsuk Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
description	Anaerobic sludge from palm oil mill effluent (POME) treatment plant was used as a source of inocula for the conversion of POME into hydrogen. Optimization of temperature and initial pH for biohydrogen production from POME was investigated by response surface methodology. Temperature of 60ºC and initial pHof 5.5 was optimized for anaerobic microflora which gave a maximum hydrogen production of 4820 ml H2/l-POME corresponding to hydrogen yield of 243 ml H2/g-sugar. Total sugar consumption and chemical oxygen demand (COD) removal efficiency were 98.7% and 46%, respectively. Long-term hydrogen production in continuous reactor at HRT of 2 days, 1 day and 12 hrs were 4850 ± 90, 4660 ± 99 and 2590 ± 120 ml H2/l-POME, respectively. Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and continuous reactors were phylogenetically related to the Thermoanaerobacterium thermosaccharolyticum. Batch reactor showed more diversity of microorganisms than continuous reactor. Microbial community structure of batch reactor was comprised of T. thermosaccharolyticum, T. bryantii, Thermoanaerobacterium sp., Clostridium thermopalmarium and Clostridium NS5-4, while continuous reactor was comprised of T. thermosaccharolyticum, T. bryantii and Thermoanaerobacterium sp. POME is good substrate for biohydrogen production under thermophilic condition with Thermoanaerobacterium species play an important role in hydrogen fermentation.
author	O-Thong,Sompong Mamimin,Chonticha Prasertsan,Poonsuk
author_facet	O-Thong,Sompong Mamimin,Chonticha Prasertsan,Poonsuk
author_sort	O-Thong,Sompong
title	Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
title_short	Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
title_full	Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
title_fullStr	Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
title_full_unstemmed	Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
title_sort	effect of temperature and initial ph on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500009
work_keys_str_mv	AT othongsompong effectoftemperatureandinitialphonbiohydrogenproductionfrompalmoilmilleffluentlongtermevaluationandmicrobialcommunityanalysis AT mamiminchonticha effectoftemperatureandinitialphonbiohydrogenproductionfrompalmoilmilleffluentlongtermevaluationandmicrobialcommunityanalysis AT prasertsanpoonsuk effectoftemperatureandinitialphonbiohydrogenproductionfrompalmoilmilleffluentlongtermevaluationandmicrobialcommunityanalysis
_version_	1718441845270249472

Effect of temperature and initial pH on biohydrogen production from palm oil mill effluent: long-term evaluation and microbial community analysis

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