Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis
As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via ge...
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Pontificia Universidad Católica de Valparaíso
2011
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oai:scielo:S0717-345820110006000012012-06-06Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesisZhang,Wei-WeiYang,Ming-MingLi,Heng-xinWang,Dun Bacillus subtilis biosynthesis integration Pglv promoter pimelic acid single crossover event As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 μg/ml VS. 198.89 μg/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 μg/ml VS. 991.59 μg/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.6 20112011-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001en |
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| language |
English |
| topic |
Bacillus subtilis biosynthesis integration Pglv promoter pimelic acid single crossover event |
| spellingShingle |
Bacillus subtilis biosynthesis integration Pglv promoter pimelic acid single crossover event Zhang,Wei-Wei Yang,Ming-Ming Li,Heng-xin Wang,Dun Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| description |
As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic acid from multiple copies integrant was about 4 times higher than that from single copy (1017.13 μg/ml VS. 198.89 μg/ml). And when induced by maltose the production of pimelic acid was about 2 times of that under non-induction conditions (2360.73 μg/ml VS. 991.59 μg/ml). Thus, these results demonstrated that the production of pimelic acid was improved obviously through reconstructed B. subtilis. It also suggested that our expression system provided a convenient source of pimelic acid that would potentially lower the cost of production of biotin from engineered B. subtilis. |
| author |
Zhang,Wei-Wei Yang,Ming-Ming Li,Heng-xin Wang,Dun |
| author_facet |
Zhang,Wei-Wei Yang,Ming-Ming Li,Heng-xin Wang,Dun |
| author_sort |
Zhang,Wei-Wei |
| title |
Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| title_short |
Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| title_full |
Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| title_fullStr |
Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| title_full_unstemmed |
Construction of recombinant Bacillus subtilis strains for efficient pimelic acid synthesis |
| title_sort |
construction of recombinant bacillus subtilis strains for efficient pimelic acid synthesis |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2011 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001 |
| work_keys_str_mv |
AT zhangweiwei constructionofrecombinantbacillussubtilisstrainsforefficientpimelicacidsynthesis AT yangmingming constructionofrecombinantbacillussubtilisstrainsforefficientpimelicacidsynthesis AT lihengxin constructionofrecombinantbacillussubtilisstrainsforefficientpimelicacidsynthesis AT wangdun constructionofrecombinantbacillussubtilisstrainsforefficientpimelicacidsynthesis |
| _version_ |
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