Expression and non-chromatographic purification of 1,3-propanediol oxidoreductase in Escherichia coli
The gene dhaT from Klebsiella pneumoniae encodes 1,3-propanediol oxidoreductase (PDOR). Thermally responsive elastin-like polypeptides (ELPs) was used as a fusion tag to purify the proteins (PDOR). The ELP gene was attached to dhaT and ligated into the pET-22b vector. Different NaCl concentrations w...
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| Autores principales: | , , , |
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2011
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600010 |
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