Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene

Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results ind...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang,Yue, Xia,Han, Yuan,Mei, Zhao,Chuanzhi, Li,Aiqin, Wang,Xingjun
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Arachis hypogaea L. cDNA library chalcone isomerase expression analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582012000100005
record_format	dspace
spelling	oai:scielo:S0717-345820120001000052012-06-06Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI geneZhang,YueXia,HanYuan,MeiZhao,ChuanzhiLi,AiqinWang,Xingjun Arachis hypogaea L. cDNA library chalcone isomerase expression analysis Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than the expression level in stem and leaf. AhCHI was expressed in a high level in seeds with a purple seed coat, while its expression was low in seed with white seed coat.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arachis hypogaea L. cDNA library chalcone isomerase expression analysis
spellingShingle	Arachis hypogaea L. cDNA library chalcone isomerase expression analysis Zhang,Yue Xia,Han Yuan,Mei Zhao,Chuanzhi Li,Aiqin Wang,Xingjun Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
description	Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than the expression level in stem and leaf. AhCHI was expressed in a high level in seeds with a purple seed coat, while its expression was low in seed with white seed coat.
author	Zhang,Yue Xia,Han Yuan,Mei Zhao,Chuanzhi Li,Aiqin Wang,Xingjun
author_facet	Zhang,Yue Xia,Han Yuan,Mei Zhao,Chuanzhi Li,Aiqin Wang,Xingjun
author_sort	Zhang,Yue
title	Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
title_short	Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
title_full	Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
title_fullStr	Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
title_full_unstemmed	Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene
title_sort	cloning and expression analysis of peanut (arachis hypogaea l.) chi gene
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005
work_keys_str_mv	AT zhangyue cloningandexpressionanalysisofpeanutarachishypogaealchigene AT xiahan cloningandexpressionanalysisofpeanutarachishypogaealchigene AT yuanmei cloningandexpressionanalysisofpeanutarachishypogaealchigene AT zhaochuanzhi cloningandexpressionanalysisofpeanutarachishypogaealchigene AT liaiqin cloningandexpressionanalysisofpeanutarachishypogaealchigene AT wangxingjun cloningandexpressionanalysisofpeanutarachishypogaealchigene
_version_	1718441850608549888

Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene

Ejemplares similares