Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines

Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred li...

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Autores principales:	González,Germán Andrés, Pacheco,María Gabriela, Oneto,Cecilia Décima, Etchart,Valeria J, Kandus,Mariana V, Salerno,Juan Carlos, Eyherabide,Guillermo, Presello,Daniel, Lewi,Dalia Marcela
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	callus inbred lines maize molecular markers regeneration somatic embryogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009
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spelling	oai:scielo:S0717-345820120001000092012-06-06Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred linesGonzález,Germán AndrésPacheco,María GabrielaOneto,Cecilia DécimaEtchart,Valeria JKandus,Mariana VSalerno,Juan CarlosEyherabide,GuillermoPresello,DanielLewi,Dalia Marcela callus inbred lines maize molecular markers regeneration somatic embryogenesis Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC (mature somatic embryos/callus evaluated) x 100], Regeneration Capacity (RC) and Fertile Plant Recovery in greenhouse (FPR, fertile plants/regenerated plants) were recorded. A total of 17 lines reached an EC > 50%, and 14 out of those 17 lines regenerated seedlings. The FPR ranged between 50 and 100%. Also, we selected three promising lines with high agronomic performance, as alternatives to Hi II, in order to be included in a maize transformation scheme via somatic embryogenesis. In addition, we report the usefulness of Single Sequences Repeat (SSRs) in the determination of genetic diversity among 14 divergent lines for somatic embryogenesis response. The seven lines displaying good in vitro behaviour can be crossed to obtain hybrids combining desirable alleles for somatic embryogenesis response and different genetic backgrounds.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	callus inbred lines maize molecular markers regeneration somatic embryogenesis
spellingShingle	callus inbred lines maize molecular markers regeneration somatic embryogenesis González,Germán Andrés Pacheco,María Gabriela Oneto,Cecilia Décima Etchart,Valeria J Kandus,Mariana V Salerno,Juan Carlos Eyherabide,Guillermo Presello,Daniel Lewi,Dalia Marcela Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
description	Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC (mature somatic embryos/callus evaluated) x 100], Regeneration Capacity (RC) and Fertile Plant Recovery in greenhouse (FPR, fertile plants/regenerated plants) were recorded. A total of 17 lines reached an EC > 50%, and 14 out of those 17 lines regenerated seedlings. The FPR ranged between 50 and 100%. Also, we selected three promising lines with high agronomic performance, as alternatives to Hi II, in order to be included in a maize transformation scheme via somatic embryogenesis. In addition, we report the usefulness of Single Sequences Repeat (SSRs) in the determination of genetic diversity among 14 divergent lines for somatic embryogenesis response. The seven lines displaying good in vitro behaviour can be crossed to obtain hybrids combining desirable alleles for somatic embryogenesis response and different genetic backgrounds.
author	González,Germán Andrés Pacheco,María Gabriela Oneto,Cecilia Décima Etchart,Valeria J Kandus,Mariana V Salerno,Juan Carlos Eyherabide,Guillermo Presello,Daniel Lewi,Dalia Marcela
author_facet	González,Germán Andrés Pacheco,María Gabriela Oneto,Cecilia Décima Etchart,Valeria J Kandus,Mariana V Salerno,Juan Carlos Eyherabide,Guillermo Presello,Daniel Lewi,Dalia Marcela
author_sort	González,Germán Andrés
title	Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
title_short	Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
title_full	Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
title_fullStr	Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
title_full_unstemmed	Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
title_sort	somatic embryogenesis and plant regeneration capacity in argentinean maize (zea mays l.) inbred lines
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009
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Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines

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