Intein-mediated expression of cecropin in Escherichia coli
Different strategies have been used to overcome the difficulties to produce antimicrobial peptides. Here we used Intein Mediated Purification with an Affinity Chitin-binding Tag (IMPACT-System, New England Biolabs) for the expression of the antimicrobial peptide cecropin to reduce its sensitivity to...
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Autores principales: | Díaz,Mauricio, Venturini,Elena, Marchetti,Stefano, Arenas,Gloria, Marshall,Sergio H |
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Lenguaje: | English |
Publicado: |
Pontificia Universidad Católica de Valparaíso
2012
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Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200003 |
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