Association between AA-NAT gene polymorphism and reproductive performance in sheep

Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reprodu...

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Autores principales:	Ding-ping,Bai, Cheng-jiang,Yu, Yu-lin,Chen
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	AA-NAT gene polymorphism reproduction seasonality sheep
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007
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spelling	oai:scielo:S0717-345820120002000072012-06-06Association between AA-NAT gene polymorphism and reproductive performance in sheepDing-ping,BaiCheng-jiang,YuYu-lin,Chen AA-NAT gene polymorphism reproduction seasonality sheep Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was ﬁrstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the signiﬁcant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.2 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AA-NAT gene polymorphism reproduction seasonality sheep
spellingShingle	AA-NAT gene polymorphism reproduction seasonality sheep Ding-ping,Bai Cheng-jiang,Yu Yu-lin,Chen Association between AA-NAT gene polymorphism and reproductive performance in sheep
description	Arylalkylamine N-acetyltransferase (AA-NAT) is critical enzyme in Melatonin (MLT) biosynthesis for MLT regulating the animal seasonal breeding. In this study, DNA sequencing methods were applied to detect the polymorphisms of the AA-NAT gene in 179 Chinese sheep belonging to two non-seasonal reproduction breeds and two seasonal reproduction breeds. One mutation at exon 3 (NM_001009461:c.486A > G) was ﬁrstly described at the sheep AA-NAT locus. Hence, we described the SmaI PCR-RFLP method for detecting EX3 486A > G mutation, frequencies of the AA-NAT-G allele varied from 0.871 to 0.908 in two non-seasonal reproduction breeds and 0.517 to 0.578 in two seasonal reproduction breeds. The associations of SmaI polymorphism with estrus traits was analyzed in non-seasonal reproduction breeds sheep and seasonal reproduction breeds sheep, the signiﬁcant statistical results were found between them, the GG genotype frequencies was higher in non-seasonal reproduction breeds (p < 0.001), while, the GA genotype frequencies was higher in seasonal reproduction breeds (p < 0.05). Hence, the EX3 486A > G mutation could facilitate association analysis and serve as a genetic marker for Chinese sheep breeding and genetics.
author	Ding-ping,Bai Cheng-jiang,Yu Yu-lin,Chen
author_facet	Ding-ping,Bai Cheng-jiang,Yu Yu-lin,Chen
author_sort	Ding-ping,Bai
title	Association between AA-NAT gene polymorphism and reproductive performance in sheep
title_short	Association between AA-NAT gene polymorphism and reproductive performance in sheep
title_full	Association between AA-NAT gene polymorphism and reproductive performance in sheep
title_fullStr	Association between AA-NAT gene polymorphism and reproductive performance in sheep
title_full_unstemmed	Association between AA-NAT gene polymorphism and reproductive performance in sheep
title_sort	association between aa-nat gene polymorphism and reproductive performance in sheep
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200007
work_keys_str_mv	AT dingpingbai associationbetweenaanatgenepolymorphismandreproductiveperformanceinsheep AT chengjiangyu associationbetweenaanatgenepolymorphismandreproductiveperformanceinsheep AT yulinchen associationbetweenaanatgenepolymorphismandreproductiveperformanceinsheep
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Association between AA-NAT gene polymorphism and reproductive performance in sheep

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