Expression of RNA polymerase IV and V in Oryza sativa
RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral...
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120002000092012-06-06Expression of RNA polymerase IV and V in Oryza sativaMoo,Leydi de Rocio CancheGonzález,Angela KúRodríguez-Zapata,Luis CarlosSuarez,VictorCastaño,Enrique Oryza sativa RNA pol IV RNA pol V somatic embryogenesis zygotic embryogenesis RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.2 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009en |
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Scielo Chile |
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Scielo Chile |
language |
English |
topic |
Oryza sativa RNA pol IV RNA pol V somatic embryogenesis zygotic embryogenesis |
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Oryza sativa RNA pol IV RNA pol V somatic embryogenesis zygotic embryogenesis Moo,Leydi de Rocio Canche González,Angela Kú Rodríguez-Zapata,Luis Carlos Suarez,Victor Castaño,Enrique Expression of RNA polymerase IV and V in Oryza sativa |
description |
RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing. |
author |
Moo,Leydi de Rocio Canche González,Angela Kú Rodríguez-Zapata,Luis Carlos Suarez,Victor Castaño,Enrique |
author_facet |
Moo,Leydi de Rocio Canche González,Angela Kú Rodríguez-Zapata,Luis Carlos Suarez,Victor Castaño,Enrique |
author_sort |
Moo,Leydi de Rocio Canche |
title |
Expression of RNA polymerase IV and V in Oryza sativa |
title_short |
Expression of RNA polymerase IV and V in Oryza sativa |
title_full |
Expression of RNA polymerase IV and V in Oryza sativa |
title_fullStr |
Expression of RNA polymerase IV and V in Oryza sativa |
title_full_unstemmed |
Expression of RNA polymerase IV and V in Oryza sativa |
title_sort |
expression of rna polymerase iv and v in oryza sativa |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2012 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009 |
work_keys_str_mv |
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_version_ |
1718441853982867456 |