Expression of RNA polymerase IV and V in Oryza sativa

RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral...

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Autores principales: Moo,Leydi de Rocio Canche, González,Angela Kú, Rodríguez-Zapata,Luis Carlos, Suarez,Victor, Castaño,Enrique
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2012
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009
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spelling oai:scielo:S0717-345820120002000092012-06-06Expression of RNA polymerase IV and V in Oryza sativaMoo,Leydi de Rocio CancheGonzález,Angela KúRodríguez-Zapata,Luis CarlosSuarez,VictorCastaño,Enrique Oryza sativa RNA pol IV RNA pol V somatic embryogenesis zygotic embryogenesis RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.2 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009en
institution Scielo Chile
collection Scielo Chile
language English
topic Oryza sativa
RNA pol IV
RNA pol V
somatic embryogenesis
zygotic embryogenesis
spellingShingle Oryza sativa
RNA pol IV
RNA pol V
somatic embryogenesis
zygotic embryogenesis
Moo,Leydi de Rocio Canche
González,Angela Kú
Rodríguez-Zapata,Luis Carlos
Suarez,Victor
Castaño,Enrique
Expression of RNA polymerase IV and V in Oryza sativa
description RNA polymerase IV and V are principal players in the RdDM pathway, where their current study has shown interaction of several factors that control DNA silencing of intergenic regions and siRNA production. DNA silencing is an important process during cell differentiation, nuclear structure and viral control. However, RNA pol IV and V are yet to be study in model monocot systems like Oryza sativa that can provide further information on genetic silence mechanism in plats. We show the expression pattern of these polymerases in tissues extracts of Oryza sativa. Detectable amounts of these polymerases are found in specific adult plant tissues and particularly expressed during somatic embryogenesis but not during early stages of normal embryo development. The use of synthetic auxin leads to an induction of both RNA pol IV and V in scutellum tissue where nuclear localization may be required for genome reorganization and gene silencing.
author Moo,Leydi de Rocio Canche
González,Angela Kú
Rodríguez-Zapata,Luis Carlos
Suarez,Victor
Castaño,Enrique
author_facet Moo,Leydi de Rocio Canche
González,Angela Kú
Rodríguez-Zapata,Luis Carlos
Suarez,Victor
Castaño,Enrique
author_sort Moo,Leydi de Rocio Canche
title Expression of RNA polymerase IV and V in Oryza sativa
title_short Expression of RNA polymerase IV and V in Oryza sativa
title_full Expression of RNA polymerase IV and V in Oryza sativa
title_fullStr Expression of RNA polymerase IV and V in Oryza sativa
title_full_unstemmed Expression of RNA polymerase IV and V in Oryza sativa
title_sort expression of rna polymerase iv and v in oryza sativa
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2012
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200009
work_keys_str_mv AT mooleydiderociocanche expressionofrnapolymeraseivandvinoryzasativa
AT gonzalezangelaku expressionofrnapolymeraseivandvinoryzasativa
AT rodriguezzapataluiscarlos expressionofrnapolymeraseivandvinoryzasativa
AT suarezvictor expressionofrnapolymeraseivandvinoryzasativa
AT castanoenrique expressionofrnapolymeraseivandvinoryzasativa
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