Sheep 7SK promoter for short hairpin RNA expression

Background: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful biological tool for the regulation of gene expression. For the synthesis of siRNA by vector-based expression systems, several mammalian small nuclear RNA (snRNA) promoters have been cloned and shown different...

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Autores principales:	Ni,Wei, Hu,Shengwei, Hazi,Wureli, Wang,Yuanzhi, Qiao,Jun, Yan,Ren, Chen,Chuangfu
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	7SK promoter RNAi sheep shRNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200010
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spelling	oai:scielo:S0717-345820120002000102012-06-06Sheep 7SK promoter for short hairpin RNA expressionNi,WeiHu,ShengweiHazi,WureliWang,YuanzhiQiao,JunYan,RenChen,Chuangfu 7SK promoter RNAi sheep shRNA Background: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful biological tool for the regulation of gene expression. For the synthesis of siRNA by vector-based expression systems, several mammalian small nuclear RNA (snRNA) promoters have been cloned and shown different transcriptional efficiencies. Results: In this study, we identified a sheep 7SK snRNA (s7SK) promoter based on the highly conserved polymerase III promoter elements. Promoter activity was measured by promoter-driven shRNA expression to suppress expression of an exogenous reporter gene and endogenous sheep gene. Conclusions: The knock down assay demonstrated that the s7SK induced more stronger inhibition effect than human U6 and H1 promoters. The use of this native sheep 7SK promoter for shRNA expressionis an important component for development of RNAi-based gene therapy and production of transgenic animals in sheep species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.2 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	7SK promoter RNAi sheep shRNA
spellingShingle	7SK promoter RNAi sheep shRNA Ni,Wei Hu,Shengwei Hazi,Wureli Wang,Yuanzhi Qiao,Jun Yan,Ren Chen,Chuangfu Sheep 7SK promoter for short hairpin RNA expression
description	Background: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful biological tool for the regulation of gene expression. For the synthesis of siRNA by vector-based expression systems, several mammalian small nuclear RNA (snRNA) promoters have been cloned and shown different transcriptional efficiencies. Results: In this study, we identified a sheep 7SK snRNA (s7SK) promoter based on the highly conserved polymerase III promoter elements. Promoter activity was measured by promoter-driven shRNA expression to suppress expression of an exogenous reporter gene and endogenous sheep gene. Conclusions: The knock down assay demonstrated that the s7SK induced more stronger inhibition effect than human U6 and H1 promoters. The use of this native sheep 7SK promoter for shRNA expressionis an important component for development of RNAi-based gene therapy and production of transgenic animals in sheep species.
author	Ni,Wei Hu,Shengwei Hazi,Wureli Wang,Yuanzhi Qiao,Jun Yan,Ren Chen,Chuangfu
author_facet	Ni,Wei Hu,Shengwei Hazi,Wureli Wang,Yuanzhi Qiao,Jun Yan,Ren Chen,Chuangfu
author_sort	Ni,Wei
title	Sheep 7SK promoter for short hairpin RNA expression
title_short	Sheep 7SK promoter for short hairpin RNA expression
title_full	Sheep 7SK promoter for short hairpin RNA expression
title_fullStr	Sheep 7SK promoter for short hairpin RNA expression
title_full_unstemmed	Sheep 7SK promoter for short hairpin RNA expression
title_sort	sheep 7sk promoter for short hairpin rna expression
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200010
work_keys_str_mv	AT niwei sheep7skpromoterforshorthairpinrnaexpression AT hushengwei sheep7skpromoterforshorthairpinrnaexpression AT haziwureli sheep7skpromoterforshorthairpinrnaexpression AT wangyuanzhi sheep7skpromoterforshorthairpinrnaexpression AT qiaojun sheep7skpromoterforshorthairpinrnaexpression AT yanren sheep7skpromoterforshorthairpinrnaexpression AT chenchuangfu sheep7skpromoterforshorthairpinrnaexpression
_version_	1718441854164271104

Sheep 7SK promoter for short hairpin RNA expression

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