Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 2...

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Autores principales:	Lin,Ling, Kan,Xianzhao, Yan,Hao, Wang,Danni
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Bacillus thuringiensis cellulase cellulose-degrading characterization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002
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spelling	oai:scielo:S0717-345820120003000022012-06-06Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strainsLin,LingKan,XianzhaoYan,HaoWang,Danni Bacillus thuringiensis cellulase cellulose-degrading characterization The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.3 20122012-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus thuringiensis cellulase cellulose-degrading characterization
spellingShingle	Bacillus thuringiensis cellulase cellulose-degrading characterization Lin,Ling Kan,Xianzhao Yan,Hao Wang,Danni Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
description	The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28ºC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40ºC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60ºC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70ºC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars.
author	Lin,Ling Kan,Xianzhao Yan,Hao Wang,Danni
author_facet	Lin,Ling Kan,Xianzhao Yan,Hao Wang,Danni
author_sort	Lin,Ling
title	Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
title_short	Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
title_full	Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
title_fullStr	Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
title_full_unstemmed	Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains
title_sort	characterization of extracellular cellulose-degrading enzymes from bacillus thuringiensis strains
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300002
work_keys_str_mv	AT linling characterizationofextracellularcellulosedegradingenzymesfrombacillusthuringiensisstrains AT kanxianzhao characterizationofextracellularcellulosedegradingenzymesfrombacillusthuringiensisstrains AT yanhao characterizationofextracellularcellulosedegradingenzymesfrombacillusthuringiensisstrains AT wangdanni characterizationofextracellularcellulosedegradingenzymesfrombacillusthuringiensisstrains
_version_	1718441854712676352

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

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