Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum

Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahmed,Sibtain, Imdad,Syeda Sana, Jamil,Amer
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	C. thermophilum kinetics T. harzianum xylanase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582012000300003
record_format	dspace
spelling	oai:scielo:S0717-345820120003000032012-06-06Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilumAhmed,SibtainImdad,Syeda SanaJamil,Amer C. thermophilum kinetics T. harzianum xylanase Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T. harzianum using oat spelt xylan as a substrate were 4.8 mg mL-1 and 0.526 µmol min-1 mg-1, respectively. Whereas values of the partially purified xylanase from C. thermophilum were 2.96 mg mL-1 and 0.25 µmol min-1 mg-1, respectively. These findings in this study have great implications for the future applications of xylanases.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.3 20122012-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	C. thermophilum kinetics T. harzianum xylanase
spellingShingle	C. thermophilum kinetics T. harzianum xylanase Ahmed,Sibtain Imdad,Syeda Sana Jamil,Amer Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
description	Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T. harzianum using oat spelt xylan as a substrate were 4.8 mg mL-1 and 0.526 µmol min-1 mg-1, respectively. Whereas values of the partially purified xylanase from C. thermophilum were 2.96 mg mL-1 and 0.25 µmol min-1 mg-1, respectively. These findings in this study have great implications for the future applications of xylanases.
author	Ahmed,Sibtain Imdad,Syeda Sana Jamil,Amer
author_facet	Ahmed,Sibtain Imdad,Syeda Sana Jamil,Amer
author_sort	Ahmed,Sibtain
title	Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
title_short	Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
title_full	Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
title_fullStr	Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
title_full_unstemmed	Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum
title_sort	comparative study for the kinetics of extracellular xylanases from trichoderma harzianum and chaetomium thermophilum
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003
work_keys_str_mv	AT ahmedsibtain comparativestudyforthekineticsofextracellularxylanasesfromtrichodermaharzianumandchaetomiumthermophilum AT imdadsyedasana comparativestudyforthekineticsofextracellularxylanasesfromtrichodermaharzianumandchaetomiumthermophilum AT jamilamer comparativestudyforthekineticsofextracellularxylanasesfromtrichodermaharzianumandchaetomiumthermophilum
_version_	1718441854944411648

Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum

Ejemplares similares