Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production

Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS...

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Autores principales:	Westman,Johan O, Taherzadeh,Mohammad J, Franzén,Carl Johan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	biofuel furfural lignocellulose phenolic inhibitors Saccharomyces cerevisiae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300005
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spelling	oai:scielo:S0717-345820120003000052012-06-06Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol productionWestman,Johan OTaherzadeh,Mohammad JFranzén,Carl Johan biofuel furfural lignocellulose phenolic inhibitors Saccharomyces cerevisiae Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to lignocellulose-derived inhibitors, was generally smaller in CCUG53310 than in CBS8066 in inhibitory media. The flocculation of CCUG53310 was linked to the expression of FLO8, FLO10 and one or more of FLO1, FLO5 or FLO9. Flocculation depended on cell wall proteins and Ca2+ ions, but was almost unaffected by other compounds and pH values typical for lignocellulosic media. Conclusions: S. cerevisiae CCUG53310 can be characterised as being very robust, with great potential for industrial fermentation of lignocellulosic hydrolysates relatively low in phenolic inhibitors.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.3 20122012-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biofuel furfural lignocellulose phenolic inhibitors Saccharomyces cerevisiae
spellingShingle	biofuel furfural lignocellulose phenolic inhibitors Saccharomyces cerevisiae Westman,Johan O Taherzadeh,Mohammad J Franzén,Carl Johan Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
description	Background: Robust second generation bioethanol processes require microorganisms able to ferment inhibitory lignocellullosic hydrolysates. In this study, the inhibitor tolerance and flocculation characteristics of Saccharomyces cerevisiae CCUG53310 were evaluated in comparison with S. cerevisiae CBS8066. Results: The flocculating strain CCUG53310 could rapidly ferment all hexoses in dilute acid spruce hydrolysate, while CBS8066 was strongly inhibited in this medium. In synthetic inhibitory media, CCUG53310 was more tolerant to carboxylic acids and furan aldehydes, but more sensitive than CBS8066 to phenolic compounds. Despite the higher tolerance, the increase in expression of the YAP1, ATR1 and FLR1 genes, known to confer resistance to lignocellulose-derived inhibitors, was generally smaller in CCUG53310 than in CBS8066 in inhibitory media. The flocculation of CCUG53310 was linked to the expression of FLO8, FLO10 and one or more of FLO1, FLO5 or FLO9. Flocculation depended on cell wall proteins and Ca2+ ions, but was almost unaffected by other compounds and pH values typical for lignocellulosic media. Conclusions: S. cerevisiae CCUG53310 can be characterised as being very robust, with great potential for industrial fermentation of lignocellulosic hydrolysates relatively low in phenolic inhibitors.
author	Westman,Johan O Taherzadeh,Mohammad J Franzén,Carl Johan
author_facet	Westman,Johan O Taherzadeh,Mohammad J Franzén,Carl Johan
author_sort	Westman,Johan O
title	Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
title_short	Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
title_full	Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
title_fullStr	Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
title_full_unstemmed	Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
title_sort	inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300005
work_keys_str_mv	AT westmanjohano inhibitortoleranceandflocculationofayeaststrainsuitableforsecondgenerationbioethanolproduction AT taherzadehmohammadj inhibitortoleranceandflocculationofayeaststrainsuitableforsecondgenerationbioethanolproduction AT franzencarljohan inhibitortoleranceandflocculationofayeaststrainsuitableforsecondgenerationbioethanolproduction
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Inhibitor tolerance and flocculation of a yeast strain suitable for second generation bioethanol production

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