Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates

Background: Unlike petroleum-based synthetic plastics, biodegradable biopolymer generation from industrial residue is a key strategy to reduce costs in the production process, as well as in the waste management, since efficient industrial wastewater treatment could be costly. In this context, the pr...

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Autores principales:	Krueger,Cristhiane L, Radetski,Claudemir M, Bendia,Amanda G, Oliveira,Ida M, Castro-Silva,Marcus A, Rambo,Carlos R, Antonio,Regina V, Lima,André O.S.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Bacillus megaterium biodegradable plastics biopolymer MTT assay starch substrates
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300008
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spelling	oai:scielo:S0717-345820120003000082012-06-06Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoatesKrueger,Cristhiane LRadetski,Claudemir MBendia,Amanda GOliveira,Ida MCastro-Silva,Marcus ARambo,Carlos RAntonio,Regina VLima,André O.S. Bacillus megaterium biodegradable plastics biopolymer MTT assay starch substrates Background: Unlike petroleum-based synthetic plastics, biodegradable biopolymer generation from industrial residue is a key strategy to reduce costs in the production process, as well as in the waste management, since efficient industrial wastewater treatment could be costly. In this context, the present work describes the prospection and use of bacterial strains capable to bioconvert cassava starch by-product into biodegradable polyhydroxyalkanoates (PHAs). Results: The first step of this study was the bacterial competence screening which was conducted with 72 strains covering 21 Bacillus and related species. The microorganism growth in a medium with a starch substrate was measured by an innovative MTT assay, while the ability of the bacteria to secrete amylase and produce PHA was evaluated by the Nile Red Dye method. Based on growth and potential for PHA production, four isolates were selected and identified as Bacillus megaterium by 16S rRNA sequencing. When cultivated in hydrolyzed cassava starch by-product, maximum production reached 4.97 g dry biomass/L with 29.7% of Poly-(3-hydroxybutyrate) (characterized by FTIR). Conclusions: MTT assay proved to be a reliable methodology for monitoring bacterial growth in insoluble media. Selected amylolytic strains could be used as an alternative industrial process for biodegradable plastics production from starchy residues, reducing costs for biodegradable biopolymer production and wastewater treatment operations.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.3 20122012-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacillus megaterium biodegradable plastics biopolymer MTT assay starch substrates
spellingShingle	Bacillus megaterium biodegradable plastics biopolymer MTT assay starch substrates Krueger,Cristhiane L Radetski,Claudemir M Bendia,Amanda G Oliveira,Ida M Castro-Silva,Marcus A Rambo,Carlos R Antonio,Regina V Lima,André O.S. Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
description	Background: Unlike petroleum-based synthetic plastics, biodegradable biopolymer generation from industrial residue is a key strategy to reduce costs in the production process, as well as in the waste management, since efficient industrial wastewater treatment could be costly. In this context, the present work describes the prospection and use of bacterial strains capable to bioconvert cassava starch by-product into biodegradable polyhydroxyalkanoates (PHAs). Results: The first step of this study was the bacterial competence screening which was conducted with 72 strains covering 21 Bacillus and related species. The microorganism growth in a medium with a starch substrate was measured by an innovative MTT assay, while the ability of the bacteria to secrete amylase and produce PHA was evaluated by the Nile Red Dye method. Based on growth and potential for PHA production, four isolates were selected and identified as Bacillus megaterium by 16S rRNA sequencing. When cultivated in hydrolyzed cassava starch by-product, maximum production reached 4.97 g dry biomass/L with 29.7% of Poly-(3-hydroxybutyrate) (characterized by FTIR). Conclusions: MTT assay proved to be a reliable methodology for monitoring bacterial growth in insoluble media. Selected amylolytic strains could be used as an alternative industrial process for biodegradable plastics production from starchy residues, reducing costs for biodegradable biopolymer production and wastewater treatment operations.
author	Krueger,Cristhiane L Radetski,Claudemir M Bendia,Amanda G Oliveira,Ida M Castro-Silva,Marcus A Rambo,Carlos R Antonio,Regina V Lima,André O.S.
author_facet	Krueger,Cristhiane L Radetski,Claudemir M Bendia,Amanda G Oliveira,Ida M Castro-Silva,Marcus A Rambo,Carlos R Antonio,Regina V Lima,André O.S.
author_sort	Krueger,Cristhiane L
title	Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
title_short	Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
title_full	Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
title_fullStr	Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
title_full_unstemmed	Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates
title_sort	bioconversion of cassava starch by-product into bacillus and related bacteria polyhydroxyalkanoates
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300008
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Bioconversion of cassava starch by-product into Bacillus and related bacteria polyhydroxyalkanoates

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