Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants

Development of quantitative competitive PCR for determination of copy number and expression level of the synthetic glyphosate oxidoreductase gene in transgenic canola plants

Background: For successful in vitro plant regeneration, plant cell lines with multiple transgene integration and low transgene expression levels need to be ruled out. Although real-time polymerase chain reaction (RT-PCR) is a rapid way to accomplish this, it is also expensive and typically limits th...

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Bibliographic Details
Main Authors: Hadi,Faranak, Salmanian,Ali Hatef, Ghazizadeh,Elham, Amani,Jafar, Noghabi,Kambiz Akbari, Mousavi,Amir
Language:English
Published: Pontificia Universidad Católica de Valparaíso 2012
Subjects:
Brassica napus L.
competitive quantitative PCR
transcript level
transgene copy number
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400002
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