Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers

Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with...

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Autores principales:	Osorio,Mayra, Gámez,Elizabeth, Molina,Sandy, Infante,Diógenes
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	AFLP genetic stability ISTR SSR UPGMA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003
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spelling	oai:scielo:S0717-345820120004000032012-08-22Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markersOsorio,MayraGámez,ElizabethMolina,SandyInfante,Diógenes AFLP genetic stability ISTR SSR UPGMA Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the beginning of the somatic embryogenesis process were grouped as independent of the other plants when analyzed at the acclimatization stage. The differences found with the different markers used are discussed. In field trials, micropropagated plants had a yield between 4 and 5 times the average yield of cassava in Venezuela. Conclusion: Despite variability in terms of DNA markers, somatic embryogenesis is suitable for mass propagation of highly performing cassava clones.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.4 20122012-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AFLP genetic stability ISTR SSR UPGMA
spellingShingle	AFLP genetic stability ISTR SSR UPGMA Osorio,Mayra Gámez,Elizabeth Molina,Sandy Infante,Diógenes Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
description	Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the beginning of the somatic embryogenesis process were grouped as independent of the other plants when analyzed at the acclimatization stage. The differences found with the different markers used are discussed. In field trials, micropropagated plants had a yield between 4 and 5 times the average yield of cassava in Venezuela. Conclusion: Despite variability in terms of DNA markers, somatic embryogenesis is suitable for mass propagation of highly performing cassava clones.
author	Osorio,Mayra Gámez,Elizabeth Molina,Sandy Infante,Diógenes
author_facet	Osorio,Mayra Gámez,Elizabeth Molina,Sandy Infante,Diógenes
author_sort	Osorio,Mayra
title	Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
title_short	Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
title_full	Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
title_fullStr	Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
title_full_unstemmed	Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
title_sort	evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003
work_keys_str_mv	AT osoriomayra evaluationofcassavaplantsgeneratedbysomaticembryogenesisatdifferentstagesofdevelopmentusingmolecularmarkers AT gamezelizabeth evaluationofcassavaplantsgeneratedbysomaticembryogenesisatdifferentstagesofdevelopmentusingmolecularmarkers AT molinasandy evaluationofcassavaplantsgeneratedbysomaticembryogenesisatdifferentstagesofdevelopmentusingmolecularmarkers AT infantediogenes evaluationofcassavaplantsgeneratedbysomaticembryogenesisatdifferentstagesofdevelopmentusingmolecularmarkers
_version_	1718441857411710976

Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers

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