Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology
Response surface methodology was used to optimize the fermentation conditions for the production of pristinamycin by immobilization of Streptomyces pristinaespiralis F213 in shaking flask cultivation. Seed medium volume, fermentation medium volume and shaking speed of seed culture were fou...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120004000062012-08-22Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodologyZhang,Li-JingZheng,· XiJin,· Zhi-HuaHu,· ShengHe,· Mei-Ru fermentation conditions immobilization pristinamycin response surface methodology Streptomyces pristinaespiralis Response surface methodology was used to optimize the fermentation conditions for the production of pristinamycin by immobilization of Streptomyces pristinaespiralis F213 in shaking flask cultivation. Seed medium volume, fermentation medium volume and shaking speed of seed culture were found to have significant effects on pristinamycin production by the Plackett-Burman design. The steepest ascent method was adopted to approach the vicinity of optimum space, followed by central composite design for further optimization. A quadratic model was built to fit the pristinamycin production. The optimum conditions were found to be seed medium volume of 29.5 ml, fermentation medium volume of 28.8 ml, and shaking speed of seed culture at 204 rpm. At the optimum conditions, a production of 213 mg/l was obtained, which was in agreement with the maximum predicted pristinamycin yield of 209 mg/l. This is the first report on pristinamycins production by immobilized S. pristinaespiralis using response surface methodology.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.4 20122012-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400006en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
fermentation conditions immobilization pristinamycin response surface methodology Streptomyces pristinaespiralis |
| spellingShingle |
fermentation conditions immobilization pristinamycin response surface methodology Streptomyces pristinaespiralis Zhang,Li-Jing Zheng,· Xi Jin,· Zhi-Hua Hu,· Sheng He,· Mei-Ru Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| description |
Response surface methodology was used to optimize the fermentation conditions for the production of pristinamycin by immobilization of Streptomyces pristinaespiralis F213 in shaking flask cultivation. Seed medium volume, fermentation medium volume and shaking speed of seed culture were found to have significant effects on pristinamycin production by the Plackett-Burman design. The steepest ascent method was adopted to approach the vicinity of optimum space, followed by central composite design for further optimization. A quadratic model was built to fit the pristinamycin production. The optimum conditions were found to be seed medium volume of 29.5 ml, fermentation medium volume of 28.8 ml, and shaking speed of seed culture at 204 rpm. At the optimum conditions, a production of 213 mg/l was obtained, which was in agreement with the maximum predicted pristinamycin yield of 209 mg/l. This is the first report on pristinamycins production by immobilized S. pristinaespiralis using response surface methodology. |
| author |
Zhang,Li-Jing Zheng,· Xi Jin,· Zhi-Hua Hu,· Sheng He,· Mei-Ru |
| author_facet |
Zhang,Li-Jing Zheng,· Xi Jin,· Zhi-Hua Hu,· Sheng He,· Mei-Ru |
| author_sort |
Zhang,Li-Jing |
| title |
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| title_short |
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| title_full |
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| title_fullStr |
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| title_full_unstemmed |
Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology |
| title_sort |
optimization of fermentation conditions for pristinamycin production by immobilized streptomyces pristinaespiralis using response surface methodology |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400006 |
| work_keys_str_mv |
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