Callus induction and plant regeneration of Ulex europaeus
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of...
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120004000072012-08-22Callus induction and plant regeneration of Ulex europaeusRamírez,IngridDorta,FernandoCuadros-Inostroza,ÁlvaroPeña-Cortés,Hugo antioxidants callus induction Fabaceae gorse plant regeneration Ulex europaeus A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.4 20122012-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400007en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
antioxidants callus induction Fabaceae gorse plant regeneration Ulex europaeus |
| spellingShingle |
antioxidants callus induction Fabaceae gorse plant regeneration Ulex europaeus Ramírez,Ingrid Dorta,Fernando Cuadros-Inostroza,Álvaro Peña-Cortés,Hugo Callus induction and plant regeneration of Ulex europaeus |
| description |
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite. |
| author |
Ramírez,Ingrid Dorta,Fernando Cuadros-Inostroza,Álvaro Peña-Cortés,Hugo |
| author_facet |
Ramírez,Ingrid Dorta,Fernando Cuadros-Inostroza,Álvaro Peña-Cortés,Hugo |
| author_sort |
Ramírez,Ingrid |
| title |
Callus induction and plant regeneration of Ulex europaeus |
| title_short |
Callus induction and plant regeneration of Ulex europaeus |
| title_full |
Callus induction and plant regeneration of Ulex europaeus |
| title_fullStr |
Callus induction and plant regeneration of Ulex europaeus |
| title_full_unstemmed |
Callus induction and plant regeneration of Ulex europaeus |
| title_sort |
callus induction and plant regeneration of ulex europaeus |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400007 |
| work_keys_str_mv |
AT ramirezingrid callusinductionandplantregenerationofulexeuropaeus AT dortafernando callusinductionandplantregenerationofulexeuropaeus AT cuadrosinostrozaalvaro callusinductionandplantregenerationofulexeuropaeus AT penacorteshugo callusinductionandplantregenerationofulexeuropaeus |
| _version_ |
1718441858458189824 |