Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor

Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use...

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Autores principales:	Sequeida,Álvaro, Tapia,Eduardo, Ortega,Marcelo, Zamora,Pablo, Castro,Álvaro, Montes,Christian, Zúñiga,Gustavo E, Prieto,Humberto
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Deschampsia antarctica phenolic compounds temporary immersion bioreactor UV-B elicitation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008
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spelling	oai:scielo:S0717-345820120004000082012-08-22Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactorSequeida,ÁlvaroTapia,EduardoOrtega,MarceloZamora,PabloCastro,ÁlvaroMontes,ChristianZúñiga,Gustavo EPrieto,Humberto Deschampsia antarctica phenolic compounds temporary immersion bioreactor UV-B elicitation Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.4 20122012-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Deschampsia antarctica phenolic compounds temporary immersion bioreactor UV-B elicitation
spellingShingle	Deschampsia antarctica phenolic compounds temporary immersion bioreactor UV-B elicitation Sequeida,Álvaro Tapia,Eduardo Ortega,Marcelo Zamora,Pablo Castro,Álvaro Montes,Christian Zúñiga,Gustavo E Prieto,Humberto Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
description	Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity were observed after 14 day of culturing. The addition of UV-B radiation pulses for 0.5 hrs at 6 hrs intervals increased total phenolics and antioxidant activity more than 3- and 1.5- fold, respectively, compared to controls with no UV-B. Significant accumulation of scopoletin, chlorogenic acid, gallic acid and rutin was found in these plantlets. This is the first bioreactor designed to optimize biomass and phenylpropanoid production in DA.
author	Sequeida,Álvaro Tapia,Eduardo Ortega,Marcelo Zamora,Pablo Castro,Álvaro Montes,Christian Zúñiga,Gustavo E Prieto,Humberto
author_facet	Sequeida,Álvaro Tapia,Eduardo Ortega,Marcelo Zamora,Pablo Castro,Álvaro Montes,Christian Zúñiga,Gustavo E Prieto,Humberto
author_sort	Sequeida,Álvaro
title	Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
title_short	Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
title_full	Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
title_fullStr	Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
title_full_unstemmed	Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor
title_sort	production of phenolic metabolites by deschampsia antarctica shoots using uv-b treatments during cultivation in a photobioreactor
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008
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Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor

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