Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum

Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate...

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Autores principales:	Ding,Yu Fei, Wang,Chuan Tang, Tang,Yue Yi, Wang,Xiu Zhen, Wu,Qi, Hu,Dong Qing, Yu,Hong Tao, Zhang,Jian Cheng, Cui,Feng Gao, Song,Guo Sheng, Gao,Hua Yuan, Yu,Shan Lin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Arachis hypogaea ARF cyclophilin differentially expressed genes genefishing Ralstonia solanacearum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500001
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spelling	oai:scielo:S0717-345820120005000012012-11-29Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearumDing,Yu FeiWang,Chuan TangTang,Yue YiWang,Xiu ZhenWu,QiHu,Dong QingYu,Hong TaoZhang,Jian ChengCui,Feng GaoSong,Guo ShengGao,Hua YuanYu,Shan Lin Arachis hypogaea ARF cyclophilin differentially expressed genes genefishing Ralstonia solanacearum Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively, were further studied by real time RT-PCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500001en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arachis hypogaea ARF cyclophilin differentially expressed genes genefishing Ralstonia solanacearum
spellingShingle	Arachis hypogaea ARF cyclophilin differentially expressed genes genefishing Ralstonia solanacearum Ding,Yu Fei Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Wu,Qi Hu,Dong Qing Yu,Hong Tao Zhang,Jian Cheng Cui,Feng Gao Song,Guo Sheng Gao,Hua Yuan Yu,Shan Lin Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
description	Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively, were further studied by real time RT-PCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.
author	Ding,Yu Fei Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Wu,Qi Hu,Dong Qing Yu,Hong Tao Zhang,Jian Cheng Cui,Feng Gao Song,Guo Sheng Gao,Hua Yuan Yu,Shan Lin
author_facet	Ding,Yu Fei Wang,Chuan Tang Tang,Yue Yi Wang,Xiu Zhen Wu,Qi Hu,Dong Qing Yu,Hong Tao Zhang,Jian Cheng Cui,Feng Gao Song,Guo Sheng Gao,Hua Yuan Yu,Shan Lin
author_sort	Ding,Yu Fei
title	Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
title_short	Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
title_full	Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
title_fullStr	Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
title_full_unstemmed	Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
title_sort	isolation and analysis of differentially expressed genes from peanut in response to challenge with ralstonia solanacearum
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500001
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Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum

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