Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments

The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits Maradol were randomly d...

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Autores principales:	Rivera-Domínguez,Marisela, Astorga-Cienfuegos,Karen Rosalinda, Tiznado-Hernández,Martin Ernesto, González-Aguilar,Gustavo Adolfo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	cold treatment defence proteins genetic expression jasmonic acid methyl ester papaya fruit
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500006
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spelling	oai:scielo:S0717-345820120005000062012-11-29Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatmentsRivera-Domínguez,MariselaAstorga-Cienfuegos,Karen RosalindaTiznado-Hernández,Martin ErnestoGonzález-Aguilar,Gustavo Adolfo cold treatment defence proteins genetic expression jasmonic acid methyl ester papaya fruit The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits Maradol were randomly divided into two groups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin tissue, the expression levels of the pdf1.1 and pdf1.2 genes were higher immediately after MJ treatment compared to the control. Furthermore, the expression of pdf1.2 remained high after MJ treatment and subsequent storage compared to the control. It was therefore concluded that the activation of the pdf1.1 and pdf1.2 genes forms part of the molecular defence mechanism in fruits that is activated by exposure to MJ. To our knowledge, this is the first study that analyzes the gene expression in papaya fruit that is induced by the exogenous application of methyl jasmonate and cold treatment.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500006en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cold treatment defence proteins genetic expression jasmonic acid methyl ester papaya fruit
spellingShingle	cold treatment defence proteins genetic expression jasmonic acid methyl ester papaya fruit Rivera-Domínguez,Marisela Astorga-Cienfuegos,Karen Rosalinda Tiznado-Hernández,Martin Ernesto González-Aguilar,Gustavo Adolfo Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
description	The defence mechanisms that are activated by methyl jasmonate (MJ) in fruits are not well understood. In this work, we studied the expression of defence genes in papaya fruit that are induced by the exposure to MJ and/or low temperatures. The papaya fruits Maradol were randomly divided into two groups: one group was the untreated control and the other was treated with 10-4 M of MJ. Half of the fruits from each of the two groups were stored after treatment for 5 days at 5ºC and 2 days at 20ºC. We studied the expression levels of the pdf1.1 and pdf1.2 genes by amplification from expression libraries created from the pulp and skin tissues of the papaya fruit. As a reference, the mRNA level of the 18s ribosomal gene was used. In the skin tissue, the expression levels of the pdf1.1 and pdf1.2 genes were higher immediately after MJ treatment compared to the control. Furthermore, the expression of pdf1.2 remained high after MJ treatment and subsequent storage compared to the control. It was therefore concluded that the activation of the pdf1.1 and pdf1.2 genes forms part of the molecular defence mechanism in fruits that is activated by exposure to MJ. To our knowledge, this is the first study that analyzes the gene expression in papaya fruit that is induced by the exogenous application of methyl jasmonate and cold treatment.
author	Rivera-Domínguez,Marisela Astorga-Cienfuegos,Karen Rosalinda Tiznado-Hernández,Martin Ernesto González-Aguilar,Gustavo Adolfo
author_facet	Rivera-Domínguez,Marisela Astorga-Cienfuegos,Karen Rosalinda Tiznado-Hernández,Martin Ernesto González-Aguilar,Gustavo Adolfo
author_sort	Rivera-Domínguez,Marisela
title	Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
title_short	Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
title_full	Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
title_fullStr	Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
title_full_unstemmed	Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments
title_sort	induction of the expression of defence genes in carica papaya fruit by methyl jasmonate and low temperature treatments
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500006
work_keys_str_mv	AT riveradominguezmarisela inductionoftheexpressionofdefencegenesincaricapapayafruitbymethyljasmonateandlowtemperaturetreatments AT astorgacienfuegoskarenrosalinda inductionoftheexpressionofdefencegenesincaricapapayafruitbymethyljasmonateandlowtemperaturetreatments AT tiznadohernandezmartinernesto inductionoftheexpressionofdefencegenesincaricapapayafruitbymethyljasmonateandlowtemperaturetreatments AT gonzalezaguilargustavoadolfo inductionoftheexpressionofdefencegenesincaricapapayafruitbymethyljasmonateandlowtemperaturetreatments
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Induction of the expression of defence genes in Carica papaya fruit by methyl jasmonate and low temperature treatments

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