A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)

In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the se...

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Autores principales:	Diez-de-Medina,Sergio, Silva,Herman
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	bin mapping molecular markers Prunus Rosaceae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500007
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spelling	oai:scielo:S0717-345820120005000072012-11-29A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)Diez-de-Medina,SergioSilva,Herman bin mapping molecular markers Prunus Rosaceae In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the search for new molecular markers that could be associated with economically important traits. Therefore, a functional approach was employed to associate candidate genes with an informative marker in peach. The data was obtained from the results of an in silico analysis of four different cold peach treatments. Thirty two candidate genes were selected that were aligned against Arabidopsis thaliana genomic sequences to design intron-flanking EST-PCR markers. These markers were used to position the candidate genes on the Prunus genetic reference map. In the physiological response to chilling injury, cell wall integrity, carbohydrate metabolism and stress response pathways could be involved, therefore candidate genes associated by Gene Ontology annotation to these pathways were included in the analysis. The designed markers were positioned to the Texas X Earlygold (TxE) genetic reference map through selective mapping methodology (Bin mapping). 72% of these new markers showed polymorphism in the TxE Binset population and 31% of them were successfully mapped to a genetic position on the Prunus reference map. The bioinformatic methodology used in this work includes a first approach in search for functional molecular markers associated to differentially expressed genes under certain physiological condition which in addition to the Bin mapping approach allows addressing a genetically anchored position to these new markers.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bin mapping molecular markers Prunus Rosaceae
spellingShingle	bin mapping molecular markers Prunus Rosaceae Diez-de-Medina,Sergio Silva,Herman A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
description	In Peach (Prunus persica) several physiological changes, such as woolliness, triggered by chilling injury are involved in major production losses due to cold storage of the fruits during shipping. Additionally, the low level of polymorphisms among peach varieties is an important limitation in the search for new molecular markers that could be associated with economically important traits. Therefore, a functional approach was employed to associate candidate genes with an informative marker in peach. The data was obtained from the results of an in silico analysis of four different cold peach treatments. Thirty two candidate genes were selected that were aligned against Arabidopsis thaliana genomic sequences to design intron-flanking EST-PCR markers. These markers were used to position the candidate genes on the Prunus genetic reference map. In the physiological response to chilling injury, cell wall integrity, carbohydrate metabolism and stress response pathways could be involved, therefore candidate genes associated by Gene Ontology annotation to these pathways were included in the analysis. The designed markers were positioned to the Texas X Earlygold (TxE) genetic reference map through selective mapping methodology (Bin mapping). 72% of these new markers showed polymorphism in the TxE Binset population and 31% of them were successfully mapped to a genetic position on the Prunus reference map. The bioinformatic methodology used in this work includes a first approach in search for functional molecular markers associated to differentially expressed genes under certain physiological condition which in addition to the Bin mapping approach allows addressing a genetically anchored position to these new markers.
author	Diez-de-Medina,Sergio Silva,Herman
author_facet	Diez-de-Medina,Sergio Silva,Herman
author_sort	Diez-de-Medina,Sergio
title	A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
title_short	A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
title_full	A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
title_fullStr	A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
title_full_unstemmed	A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)
title_sort	molecular marker approach using intron flanking est-pcr to map candidate genes in peach (prunus persica)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500007
work_keys_str_mv	AT diezdemedinasergio amolecularmarkerapproachusingintronflankingestpcrtomapcandidategenesinpeachprunuspersica AT silvaherman amolecularmarkerapproachusingintronflankingestpcrtomapcandidategenesinpeachprunuspersica AT diezdemedinasergio molecularmarkerapproachusingintronflankingestpcrtomapcandidategenesinpeachprunuspersica AT silvaherman molecularmarkerapproachusingintronflankingestpcrtomapcandidategenesinpeachprunuspersica
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A molecular marker approach using intron flanking EST-PCR to map candidate genes in peach (Prunus persica)

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