Analysis of genetic variability by ISSR markers in Calibrachoa caesia

Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and on...

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Autores principales:	Pérez de la Torre,Mariana, García,Martín, Heinz,Ruth, Escandón,Alejandro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	anchored microsatellites molecular markers ornamental plants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500008
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spelling	oai:scielo:S0717-345820120005000082012-11-29Analysis of genetic variability by ISSR markers in Calibrachoa caesiaPérez de la Torre,MarianaGarcía,MartínHeinz,RuthEscandón,Alejandro anchored microsatellites molecular markers ornamental plants Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5CT, 5CA, 5GA, 5GACA, 3CAC, 3TG and 3TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5GACA and 3AG primers, respectively, while the average values for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5GACA and 5GA, and the less informative was 3AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	anchored microsatellites molecular markers ornamental plants
spellingShingle	anchored microsatellites molecular markers ornamental plants Pérez de la Torre,Mariana García,Martín Heinz,Ruth Escandón,Alejandro Analysis of genetic variability by ISSR markers in Calibrachoa caesia
description	Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5CT, 5CA, 5GA, 5GACA, 3CAC, 3TG and 3TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5GACA and 3AG primers, respectively, while the average values for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5GACA and 5GA, and the less informative was 3AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.
author	Pérez de la Torre,Mariana García,Martín Heinz,Ruth Escandón,Alejandro
author_facet	Pérez de la Torre,Mariana García,Martín Heinz,Ruth Escandón,Alejandro
author_sort	Pérez de la Torre,Mariana
title	Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_short	Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_full	Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_fullStr	Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_full_unstemmed	Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_sort	analysis of genetic variability by issr markers in calibrachoa caesia
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500008
work_keys_str_mv	AT perezdelatorremariana analysisofgeneticvariabilitybyissrmarkersincalibrachoacaesia AT garciamartin analysisofgeneticvariabilitybyissrmarkersincalibrachoacaesia AT heinzruth analysisofgeneticvariabilitybyissrmarkersincalibrachoacaesia AT escandonalejandro analysisofgeneticvariabilitybyissrmarkersincalibrachoacaesia
_version_	1718441861146738688

Analysis of genetic variability by ISSR markers in Calibrachoa caesia

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