Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria

Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that...

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Autores principales:	Odisi,Estácio Jussie, Silvestrin,Marcela Bruschi, Takahashi,Rodrigo Yoji Uwamori, da Silva,Marcus Adonai Castro, Lima,André Oliveira de Souza
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	bacteria cellulases deep-sea lipases
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018
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spelling	oai:scielo:S0717-345820120005000182012-11-29Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteriaOdisi,Estácio JussieSilvestrin,Marcela BruschiTakahashi,Rodrigo Yoji Uwamorida Silva,Marcus Adonai CastroLima,André Oliveira de Souza bacteria cellulases deep-sea lipases Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at the water column. Based on the ability to produce cellulases and lipases three isolates were selected and identified (16S rRNA sequencing) as Bacillus stratosphericus, B. aerophilus and B. pumilus. Lipases of strain B. aerophilus LAMA 582 exhibited activity at a wide temperature range (4º to 37ºC) and include psychrophilic behaviour. Strain Bacillus stratosphericus LAMA 585 can growth in a rich (Luria Bertani) and minimal (Marine Minimal) medium, and does not need an inducer to produce its mesophilic cellulases and lipases. Conclusions: Deep-sea sediments have great potential for bioprospection of cellulase and lipase-producing bacteria. The strains LAMA 582 and LAMA 585 with their special features, exhibit a great potential to application at many biotechnology process.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bacteria cellulases deep-sea lipases
spellingShingle	bacteria cellulases deep-sea lipases Odisi,Estácio Jussie Silvestrin,Marcela Bruschi Takahashi,Rodrigo Yoji Uwamori da Silva,Marcus Adonai Castro Lima,André Oliveira de Souza Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
description	Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at the water column. Based on the ability to produce cellulases and lipases three isolates were selected and identified (16S rRNA sequencing) as Bacillus stratosphericus, B. aerophilus and B. pumilus. Lipases of strain B. aerophilus LAMA 582 exhibited activity at a wide temperature range (4º to 37ºC) and include psychrophilic behaviour. Strain Bacillus stratosphericus LAMA 585 can growth in a rich (Luria Bertani) and minimal (Marine Minimal) medium, and does not need an inducer to produce its mesophilic cellulases and lipases. Conclusions: Deep-sea sediments have great potential for bioprospection of cellulase and lipase-producing bacteria. The strains LAMA 582 and LAMA 585 with their special features, exhibit a great potential to application at many biotechnology process.
author	Odisi,Estácio Jussie Silvestrin,Marcela Bruschi Takahashi,Rodrigo Yoji Uwamori da Silva,Marcus Adonai Castro Lima,André Oliveira de Souza
author_facet	Odisi,Estácio Jussie Silvestrin,Marcela Bruschi Takahashi,Rodrigo Yoji Uwamori da Silva,Marcus Adonai Castro Lima,André Oliveira de Souza
author_sort	Odisi,Estácio Jussie
title	Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
title_short	Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
title_full	Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
title_fullStr	Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
title_full_unstemmed	Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria
title_sort	bioprospection of cellulolytic and lipolytic south atlantic deep-sea bacteria
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018
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Bioprospection of cellulolytic and lipolytic South Atlantic deep-sea bacteria

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