Anti-peptide antibodies: A tool for detecting IL-8 in salmonids

Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conse...

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Autores principales:	Santana,Paula, Palacios,Claudia, Narváez,Edgar, Guzmán,Fanny, Gallardo,José A, Mercado,Luis
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500020
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spelling	oai:scielo:S0717-345820120005000202012-11-29Anti-peptide antibodies: A tool for detecting IL-8 in salmonidsSantana,PaulaPalacios,ClaudiaNarváez,EdgarGuzmán,FannyGallardo,José AMercado,Luis anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar, presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and synthesized an epitope peptide of 10 residues with a purity of 95% and mass of 1158.7 kDa, which showed a random coil structure. From this peptide it was able to generate a polyclonal mono-specific antibody which was capable of detecting the whole molecule of IL-8 in tissue and cellular model of salmonids. Conclusions: The resulting antibody is a versatile tool for detecting IL-8 by different immune techniques such as ELISA, dot blot, western blotting and immunocytofluorescence. Analysis of IL-8 at proteomic level is a useful method for characterizing immune properties of this molecule in fish.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.5 20122012-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500020en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope
spellingShingle	anti-IL-8 fish antibodies fish chemokine rainbow trout synthetic peptide epitope Santana,Paula Palacios,Claudia Narváez,Edgar Guzmán,Fanny Gallardo,José A Mercado,Luis Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
description	Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar, presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and synthesized an epitope peptide of 10 residues with a purity of 95% and mass of 1158.7 kDa, which showed a random coil structure. From this peptide it was able to generate a polyclonal mono-specific antibody which was capable of detecting the whole molecule of IL-8 in tissue and cellular model of salmonids. Conclusions: The resulting antibody is a versatile tool for detecting IL-8 by different immune techniques such as ELISA, dot blot, western blotting and immunocytofluorescence. Analysis of IL-8 at proteomic level is a useful method for characterizing immune properties of this molecule in fish.
author	Santana,Paula Palacios,Claudia Narváez,Edgar Guzmán,Fanny Gallardo,José A Mercado,Luis
author_facet	Santana,Paula Palacios,Claudia Narváez,Edgar Guzmán,Fanny Gallardo,José A Mercado,Luis
author_sort	Santana,Paula
title	Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
title_short	Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
title_full	Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
title_fullStr	Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
title_full_unstemmed	Anti-peptide antibodies: A tool for detecting IL-8 in salmonids
title_sort	anti-peptide antibodies: a tool for detecting il-8 in salmonids
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500020
work_keys_str_mv	AT santanapaula antipeptideantibodiesatoolfordetectingil8insalmonids AT palaciosclaudia antipeptideantibodiesatoolfordetectingil8insalmonids AT narvaezedgar antipeptideantibodiesatoolfordetectingil8insalmonids AT guzmanfanny antipeptideantibodiesatoolfordetectingil8insalmonids AT gallardojosea antipeptideantibodiesatoolfordetectingil8insalmonids AT mercadoluis antipeptideantibodiesatoolfordetectingil8insalmonids
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Anti-peptide antibodies: A tool for detecting IL-8 in salmonids

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