Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843
Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignificat...
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120006000012013-01-14Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843Riansa-ngawong,WiboonSuwansaard,ManeewanPrasertsan,Poonsuk Candida shehatae ethanol production immobilization palm pressed fiber Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L-1, ethanol yield 0.47 g g-1) than those of free cells (ethanol production 10.8 g L-1, ethanol yield 0.44 g g-1) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600001en10.2225/vol15-issue6-fulltext-1 |
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Candida shehatae ethanol production immobilization palm pressed fiber |
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Candida shehatae ethanol production immobilization palm pressed fiber Riansa-ngawong,Wiboon Suwansaard,Maneewan Prasertsan,Poonsuk Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
description |
Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L-1, ethanol yield 0.47 g g-1) than those of free cells (ethanol production 10.8 g L-1, ethanol yield 0.44 g g-1) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843. |
author |
Riansa-ngawong,Wiboon Suwansaard,Maneewan Prasertsan,Poonsuk |
author_facet |
Riansa-ngawong,Wiboon Suwansaard,Maneewan Prasertsan,Poonsuk |
author_sort |
Riansa-ngawong,Wiboon |
title |
Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
title_short |
Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
title_full |
Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
title_fullStr |
Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
title_full_unstemmed |
Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843 |
title_sort |
application of palm pressed fiber as a carrier for ethanol production by candida shehatae tistr5843 |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2012 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600001 |
work_keys_str_mv |
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_version_ |
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