Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamst...

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Autores principales:	Vergara,Mauricio, Becerra,Silvana, Díaz-Barrera,Alvaro, Berrios,Julio, Altamirano,Claudia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	CHO cells galactose glutamate low temperature perfusion culture rh-tPA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002
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spelling	oai:scielo:S0717-345820120006000022013-01-14Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPAVergara,MauricioBecerra,SilvanaDíaz-Barrera,AlvaroBerrios,JulioAltamirano,Claudia CHO cells galactose glutamate low temperature perfusion culture rh-tPA We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to the culture at 37ºC. In semi-perfusion culture, the highest rh-tPA specific productivity was obtained at 34ºC. Similarly, whether a decrease in the temperature is accompanied of the replacement of glucose by galactose, the rh-tPA specific productivity improved about 112% over that obtained in semi-perfusion culture carried out at 37ºC with glucose as the carbon source. A semi-perfusion culture strategy was implemented based on the combined effect of the chosen carbon source and low temperatures, which was a useful approach for enhance the specific productivity of the recombinant protein.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002en10.2225/vol15-issue6-fulltext-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CHO cells galactose glutamate low temperature perfusion culture rh-tPA
spellingShingle	CHO cells galactose glutamate low temperature perfusion culture rh-tPA Vergara,Mauricio Becerra,Silvana Díaz-Barrera,Alvaro Berrios,Julio Altamirano,Claudia Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
description	We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to the culture at 37ºC. In semi-perfusion culture, the highest rh-tPA specific productivity was obtained at 34ºC. Similarly, whether a decrease in the temperature is accompanied of the replacement of glucose by galactose, the rh-tPA specific productivity improved about 112% over that obtained in semi-perfusion culture carried out at 37ºC with glucose as the carbon source. A semi-perfusion culture strategy was implemented based on the combined effect of the chosen carbon source and low temperatures, which was a useful approach for enhance the specific productivity of the recombinant protein.
author	Vergara,Mauricio Becerra,Silvana Díaz-Barrera,Alvaro Berrios,Julio Altamirano,Claudia
author_facet	Vergara,Mauricio Becerra,Silvana Díaz-Barrera,Alvaro Berrios,Julio Altamirano,Claudia
author_sort	Vergara,Mauricio
title	Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
title_short	Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
title_full	Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
title_fullStr	Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
title_full_unstemmed	Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
title_sort	simultaneous environmental manipulations in semi-perfusion cultures of cho cells producing rh-tpa
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002
work_keys_str_mv	AT vergaramauricio simultaneousenvironmentalmanipulationsinsemiperfusionculturesofchocellsproducingrhtpa AT becerrasilvana simultaneousenvironmentalmanipulationsinsemiperfusionculturesofchocellsproducingrhtpa AT diazbarreraalvaro simultaneousenvironmentalmanipulationsinsemiperfusionculturesofchocellsproducingrhtpa AT berriosjulio simultaneousenvironmentalmanipulationsinsemiperfusionculturesofchocellsproducingrhtpa AT altamiranoclaudia simultaneousenvironmentalmanipulationsinsemiperfusionculturesofchocellsproducingrhtpa
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Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA

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