Simultaneous environmental manipulations in semi-perfusion cultures of CHO cells producing rh-tPA
We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamst...
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Autores principales: | Vergara,Mauricio, Becerra,Silvana, Díaz-Barrera,Alvaro, Berrios,Julio, Altamirano,Claudia |
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Lenguaje: | English |
Publicado: |
Pontificia Universidad Católica de Valparaíso
2012
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Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600002 |
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