Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield

This study was carried out in order to verify whether natural selection acts on segregating common bean populations grown in the presence and absence of N, and to verify that natural selection affects the frequency of microsatellite alleles specifically, and in order to identify those that can assis...

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Autores principales:	Leite,Monik Evelin, Lima,Igor Almeida, Pereira,Dyanna Rangel, Santos,João Bosco dos
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	adaptation nitrogen use efficiency Phaseolus vulgaris SSR markers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600005
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spelling	oai:scielo:S0717-345820120006000052013-01-14Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yieldLeite,Monik EvelinLima,Igor AlmeidaPereira,Dyanna RangelSantos,João Bosco dos adaptation nitrogen use efficiency Phaseolus vulgaris SSR markers This study was carried out in order to verify whether natural selection acts on segregating common bean populations grown in the presence and absence of N, and to verify that natural selection affects the frequency of microsatellite alleles specifically, and in order to identify those that can assist in selection. Four populations from the crosses Ouro Negro x CI-107 and VC-5 x IAPAR-81 were carried forward in bulk to F8 at both levels of N. Ouro Negro and VC-5 are considered stress tolerant to nitrogen and IAPAR-81 and CI-107 responsive. One hundred progenies were obtained from each cross and grown in the presence and absence of N. DNA was extracted from 400 progenies, of which 194 were evaluated in the rainy season and 79 in the winter crop for grain yield. These progenies were evaluated in two separate experiments with and without N, in a 14 x 14 lattice design during rainy season and 9 x 9 in the winter season. We selected 35 pairs of polymorphic primers from the parents, with 20 being common from the two crosses. Using DNA of the 400 progenies, it was found that natural selection acted in 33 loci, in at least one environment and cross, and that alleles of the four parents were favoured by natural selection. QTLs were identified for the response rate of N and for grain yield and the linked markers are potential for assisted selection, especially the two most stable; BMD-20, in response to the use of N and PVBR-93 for grain yield.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600005en10.2225/vol15-issue6-fulltext-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	adaptation nitrogen use efficiency Phaseolus vulgaris SSR markers
spellingShingle	adaptation nitrogen use efficiency Phaseolus vulgaris SSR markers Leite,Monik Evelin Lima,Igor Almeida Pereira,Dyanna Rangel Santos,João Bosco dos Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
description	This study was carried out in order to verify whether natural selection acts on segregating common bean populations grown in the presence and absence of N, and to verify that natural selection affects the frequency of microsatellite alleles specifically, and in order to identify those that can assist in selection. Four populations from the crosses Ouro Negro x CI-107 and VC-5 x IAPAR-81 were carried forward in bulk to F8 at both levels of N. Ouro Negro and VC-5 are considered stress tolerant to nitrogen and IAPAR-81 and CI-107 responsive. One hundred progenies were obtained from each cross and grown in the presence and absence of N. DNA was extracted from 400 progenies, of which 194 were evaluated in the rainy season and 79 in the winter crop for grain yield. These progenies were evaluated in two separate experiments with and without N, in a 14 x 14 lattice design during rainy season and 9 x 9 in the winter season. We selected 35 pairs of polymorphic primers from the parents, with 20 being common from the two crosses. Using DNA of the 400 progenies, it was found that natural selection acted in 33 loci, in at least one environment and cross, and that alleles of the four parents were favoured by natural selection. QTLs were identified for the response rate of N and for grain yield and the linked markers are potential for assisted selection, especially the two most stable; BMD-20, in response to the use of N and PVBR-93 for grain yield.
author	Leite,Monik Evelin Lima,Igor Almeida Pereira,Dyanna Rangel Santos,João Bosco dos
author_facet	Leite,Monik Evelin Lima,Igor Almeida Pereira,Dyanna Rangel Santos,João Bosco dos
author_sort	Leite,Monik Evelin
title	Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
title_short	Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
title_full	Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
title_fullStr	Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
title_full_unstemmed	Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
title_sort	natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600005
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Natural selection for efficiency in the use of nitrogen in common bean identified by analysis of microsatellites and grain yield

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