Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers

Background: The Bemisia tabaci is one of the most devastating pests of agricultural crops and ornamental plants worldwide. The genetic diversity and biotype status of the Bemisia tabaci in Pakistan was assessed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total 8...

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Autores principales:	Hameed,Saima, Hameed,Shahid, Sadia,Munazza, Malik,Salman A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	Asia II 1 genetic diversity UPGMA whitefly
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600006
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spelling	oai:scielo:S0717-345820120006000062013-01-14Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markersHameed,SaimaHameed,ShahidSadia,MunazzaMalik,Salman A Asia II 1 genetic diversity UPGMA whitefly Background: The Bemisia tabaci is one of the most devastating pests of agricultural crops and ornamental plants worldwide. The genetic diversity and biotype status of the Bemisia tabaci in Pakistan was assessed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total 80 samples of B. tabaci collected from 14 districts of the Punjab province and 7 districts of the Sindh province were included. Results: All 10 primers screened in this study generated 151 scorable amplification products, of which 117 or 77% were polymorphic. Pairwise Nei and Lis similarity had ranged from 0.25 to 0.88 among all individuals analyzed. Based on Nei and Lis similarity coefficients Bemisia populations were grouped into 3 main clusters and clearly distinguished the non B biotype from the B biotype. Conclusion: The level of similarity among populations of same biotypes was high whereas between populations of non B and B biotypes appeared to be less closely related. This analysis showed that non B biotype is prevalent in both provinces however B biotype is restricted to few locations in Sindh. This monitoring of the spread of B. tabaci in Pakistan will assist in the establishment of appropriate management strategies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600006en10.2225/vol15-issue6-fulltext-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Asia II 1 genetic diversity UPGMA whitefly
spellingShingle	Asia II 1 genetic diversity UPGMA whitefly Hameed,Saima Hameed,Shahid Sadia,Munazza Malik,Salman A Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
description	Background: The Bemisia tabaci is one of the most devastating pests of agricultural crops and ornamental plants worldwide. The genetic diversity and biotype status of the Bemisia tabaci in Pakistan was assessed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total 80 samples of B. tabaci collected from 14 districts of the Punjab province and 7 districts of the Sindh province were included. Results: All 10 primers screened in this study generated 151 scorable amplification products, of which 117 or 77% were polymorphic. Pairwise Nei and Lis similarity had ranged from 0.25 to 0.88 among all individuals analyzed. Based on Nei and Lis similarity coefficients Bemisia populations were grouped into 3 main clusters and clearly distinguished the non B biotype from the B biotype. Conclusion: The level of similarity among populations of same biotypes was high whereas between populations of non B and B biotypes appeared to be less closely related. This analysis showed that non B biotype is prevalent in both provinces however B biotype is restricted to few locations in Sindh. This monitoring of the spread of B. tabaci in Pakistan will assist in the establishment of appropriate management strategies.
author	Hameed,Saima Hameed,Shahid Sadia,Munazza Malik,Salman A
author_facet	Hameed,Saima Hameed,Shahid Sadia,Munazza Malik,Salman A
author_sort	Hameed,Saima
title	Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
title_short	Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
title_full	Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
title_fullStr	Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
title_full_unstemmed	Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
title_sort	genetic diversity analysis of bemisia tabaci populations in pakistan using rapd markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600006
work_keys_str_mv	AT hameedsaima geneticdiversityanalysisofbemisiatabacipopulationsinpakistanusingrapdmarkers AT hameedshahid geneticdiversityanalysisofbemisiatabacipopulationsinpakistanusingrapdmarkers AT sadiamunazza geneticdiversityanalysisofbemisiatabacipopulationsinpakistanusingrapdmarkers AT maliksalmana geneticdiversityanalysisofbemisiatabacipopulationsinpakistanusingrapdmarkers
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Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers

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