The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields

The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw br...

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Autores principales:	Nunkaew,Tomorn, Kantachote,Duangporn, Nitoda,Teruhiko, Kanzaki,Hiroshi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	biofertilizer isolation paddy field purple nonsulfur bacteria rice straw broth
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007
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spelling	oai:scielo:S0717-345820120006000072013-01-14The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fieldsNunkaew,TomornKantachote,DuangpornNitoda,TeruhikoKanzaki,Hiroshi biofertilizer isolation paddy field purple nonsulfur bacteria rice straw broth The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw broth provided about a 2 fold increase in the number of samples that produced PNSB growth. Colonies isolated from GA broth required a significantly higher number of repeated streaking to obtain a pure culture (average 3.5) than those from rice straw broth (average 2.7) and the latter medium also produced significantly (P < 0.05) more isolates per sample. Sixty samples of water and sediment, from rice paddies with salinity (average, 3.43 ± 0.67 mS/cm) and slight acidity (average, pH 5.84 ± 0.42) provided 62 PNSB isolates by GA broth and 210 isolates by rice straw broth, and rice straw broth also produced a greater prevalence of PNSB. Estimates of the costs based on current prices of media, Gas Pak and electricity to obtain PNSB with the use of GA broth was roughly 6 times higher than for the rice straw broth.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007en10.2225/vol15-issue6-fulltext-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biofertilizer isolation paddy field purple nonsulfur bacteria rice straw broth
spellingShingle	biofertilizer isolation paddy field purple nonsulfur bacteria rice straw broth Nunkaew,Tomorn Kantachote,Duangporn Nitoda,Teruhiko Kanzaki,Hiroshi The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
description	The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw broth provided about a 2 fold increase in the number of samples that produced PNSB growth. Colonies isolated from GA broth required a significantly higher number of repeated streaking to obtain a pure culture (average 3.5) than those from rice straw broth (average 2.7) and the latter medium also produced significantly (P < 0.05) more isolates per sample. Sixty samples of water and sediment, from rice paddies with salinity (average, 3.43 ± 0.67 mS/cm) and slight acidity (average, pH 5.84 ± 0.42) provided 62 PNSB isolates by GA broth and 210 isolates by rice straw broth, and rice straw broth also produced a greater prevalence of PNSB. Estimates of the costs based on current prices of media, Gas Pak and electricity to obtain PNSB with the use of GA broth was roughly 6 times higher than for the rice straw broth.
author	Nunkaew,Tomorn Kantachote,Duangporn Nitoda,Teruhiko Kanzaki,Hiroshi
author_facet	Nunkaew,Tomorn Kantachote,Duangporn Nitoda,Teruhiko Kanzaki,Hiroshi
author_sort	Nunkaew,Tomorn
title	The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
title_short	The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
title_full	The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
title_fullStr	The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
title_full_unstemmed	The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
title_sort	use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007
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The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields

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