Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization
Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex p...
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Pontificia Universidad Católica de Valparaíso
2012
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oai:scielo:S0717-345820120006000082013-01-14Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourizationElgueta,SebastiánRubilar,OlgaLima,NelsonDiez,M. Cristina complex pellets ligninolytic enzymes RO165 decolourization white-rot fungi Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008en10.2225/vol15-issue6-fulltext-10 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
complex pellets ligninolytic enzymes RO165 decolourization white-rot fungi |
| spellingShingle |
complex pellets ligninolytic enzymes RO165 decolourization white-rot fungi Elgueta,Sebastián Rubilar,Olga Lima,Nelson Diez,M. Cristina Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| description |
Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor showed a higher enzymatic production mainly MnP (38 U L-1 at day 15) compared to coated and simple pellets. Examinations using SEM showed that both pellets produced a black core that was entrapped by a layer of fungal mycelium. Decolourization of RO165 was demonstrated with all the pellets formulated. However, the highest and fastest decolourization was obtained with complex pellets (100% at day 8). Therefore, complex pellets of A. discolor can be used for the biological treatment of wastewater contaminated with RO165. |
| author |
Elgueta,Sebastián Rubilar,Olga Lima,Nelson Diez,M. Cristina |
| author_facet |
Elgueta,Sebastián Rubilar,Olga Lima,Nelson Diez,M. Cristina |
| author_sort |
Elgueta,Sebastián |
| title |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| title_short |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| title_full |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| title_fullStr |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| title_full_unstemmed |
Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization |
| title_sort |
selection of white-rot fungi to formulate complex and coated pellets for reactive orange 165 decolourization |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008 |
| work_keys_str_mv |
AT elguetasebastian selectionofwhiterotfungitoformulatecomplexandcoatedpelletsforreactiveorange165decolourization AT rubilarolga selectionofwhiterotfungitoformulatecomplexandcoatedpelletsforreactiveorange165decolourization AT limanelson selectionofwhiterotfungitoformulatecomplexandcoatedpelletsforreactiveorange165decolourization AT diezmcristina selectionofwhiterotfungitoformulatecomplexandcoatedpelletsforreactiveorange165decolourization |
| _version_ |
1718441867030298624 |