Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)

Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The e...

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Autores principales:	Zhao,Da Qiu, Han,Chen Xia, Ge,Jin Tao, Tao,Jun
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2012
Materias:	anthocyanin expression glucosyltransferase herbaceous peony
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600009
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spelling	oai:scielo:S0717-345820120006000092013-01-14Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)Zhao,Da QiuHan,Chen XiaGe,Jin TaoTao,Jun anthocyanin expression glucosyltransferase herbaceous peony Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.15 n.6 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600009en10.2225/vol15-issue6-fulltext-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	anthocyanin expression glucosyltransferase herbaceous peony
spellingShingle	anthocyanin expression glucosyltransferase herbaceous peony Zhao,Da Qiu Han,Chen Xia Ge,Jin Tao Tao,Jun Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
description	Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering.
author	Zhao,Da Qiu Han,Chen Xia Ge,Jin Tao Tao,Jun
author_facet	Zhao,Da Qiu Han,Chen Xia Ge,Jin Tao Tao,Jun
author_sort	Zhao,Da Qiu
title	Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
title_short	Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
title_full	Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
title_fullStr	Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
title_full_unstemmed	Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
title_sort	isolation of a udp-glucose: flavonoid 5-o-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (paeonia lactiflora pall.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600009
work_keys_str_mv	AT zhaodaqiu isolationofaudpglucoseflavonoid5oglucosyltransferasegeneandexpressionanalysisofanthocyaninbiosyntheticgenesinherbaceouspeonypaeonialactiflorapall AT hanchenxia isolationofaudpglucoseflavonoid5oglucosyltransferasegeneandexpressionanalysisofanthocyaninbiosyntheticgenesinherbaceouspeonypaeonialactiflorapall AT gejintao isolationofaudpglucoseflavonoid5oglucosyltransferasegeneandexpressionanalysisofanthocyaninbiosyntheticgenesinherbaceouspeonypaeonialactiflorapall AT taojun isolationofaudpglucoseflavonoid5oglucosyltransferasegeneandexpressionanalysisofanthocyaninbiosyntheticgenesinherbaceouspeonypaeonialactiflorapall
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Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)

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