Evaluation of antimicrobial activity and cell viability of Aloe vera sponges

Background: Aloe vera L., member of the Liliaceae family, has been shown to stimulate cell proliferation and contribute to healing and angiogenesis, has anti-bacterial, anti-fungal and anti-inflammatory activity. In addition, Aloe vera can be used as a support for drug transport. Our objective is to...

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Autores principales:	Gontijo,Sávio Morato de Lacerda, Gomes,Alinne Damásia Martins, Gala-García,Alfonso, Sinisterra,Rubén Dario, Cortés,Maria Esperanza
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	amoxicillin cytotoxicity drug transport nystatin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100002
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spelling	oai:scielo:S0717-345820130001000022013-01-21Evaluation of antimicrobial activity and cell viability of Aloe vera spongesGontijo,Sávio Morato de LacerdaGomes,Alinne Damásia MartinsGala-García,AlfonsoSinisterra,Rubén DarioCortés,Maria Esperanza amoxicillin cytotoxicity drug transport nystatin Background: Aloe vera L., member of the Liliaceae family, has been shown to stimulate cell proliferation and contribute to healing and angiogenesis, has anti-bacterial, anti-fungal and anti-inflammatory activity. In addition, Aloe vera can be used as a support for drug transport. Our objective is to evaluate antimicrobial activity and cytotoxicity of sponges of Aloe vera L. for use as a carrying support of drugs. Results: In this work, sponge of free Aloe vera (AV) loaded with amoxicillin (AMX) or nystatin (NYS) at 1% w/w, were prepared and physico-chemically characterized via X-ray diffraction, Fourier Transform Infrared Spectroscopy and thermal analysis. Antimicrobial potency of AV sponge alone, loaded with AMX or NYS, against strains of Streptococcus mutans, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans, Enterococcus faecalis and Candida albicans was determined. Osteoblasts and human gingival fibroblasts were cultivated on AV, Aloe vera loaded with amoxicillin (AV/AMX) and Aloe vera loaded with nystatin (AV/NYS) and cellular viability was assessed. The physico-chemical characterization performed suggested that the loaded drugs were dispersed in the sponge and those interactions between the AV sponge and the loaded drugs were weak. Furthermore, AV loaded with AMX or NYS demonstrated antimicrobial potency and osteoblasts and fibroblasts were viable after 24 hrs on free AV, and AV loaded with AMX or NYS. Conclusions: Our results indicate that sponges of free AV, loaded with AMX or NYS, are biocompatible and exhibit antimicrobial activity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.1 20132013-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100002en10.2225/vol16-issue1-fulltext-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	amoxicillin cytotoxicity drug transport nystatin
spellingShingle	amoxicillin cytotoxicity drug transport nystatin Gontijo,Sávio Morato de Lacerda Gomes,Alinne Damásia Martins Gala-García,Alfonso Sinisterra,Rubén Dario Cortés,Maria Esperanza Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
description	Background: Aloe vera L., member of the Liliaceae family, has been shown to stimulate cell proliferation and contribute to healing and angiogenesis, has anti-bacterial, anti-fungal and anti-inflammatory activity. In addition, Aloe vera can be used as a support for drug transport. Our objective is to evaluate antimicrobial activity and cytotoxicity of sponges of Aloe vera L. for use as a carrying support of drugs. Results: In this work, sponge of free Aloe vera (AV) loaded with amoxicillin (AMX) or nystatin (NYS) at 1% w/w, were prepared and physico-chemically characterized via X-ray diffraction, Fourier Transform Infrared Spectroscopy and thermal analysis. Antimicrobial potency of AV sponge alone, loaded with AMX or NYS, against strains of Streptococcus mutans, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans, Enterococcus faecalis and Candida albicans was determined. Osteoblasts and human gingival fibroblasts were cultivated on AV, Aloe vera loaded with amoxicillin (AV/AMX) and Aloe vera loaded with nystatin (AV/NYS) and cellular viability was assessed. The physico-chemical characterization performed suggested that the loaded drugs were dispersed in the sponge and those interactions between the AV sponge and the loaded drugs were weak. Furthermore, AV loaded with AMX or NYS demonstrated antimicrobial potency and osteoblasts and fibroblasts were viable after 24 hrs on free AV, and AV loaded with AMX or NYS. Conclusions: Our results indicate that sponges of free AV, loaded with AMX or NYS, are biocompatible and exhibit antimicrobial activity.
author	Gontijo,Sávio Morato de Lacerda Gomes,Alinne Damásia Martins Gala-García,Alfonso Sinisterra,Rubén Dario Cortés,Maria Esperanza
author_facet	Gontijo,Sávio Morato de Lacerda Gomes,Alinne Damásia Martins Gala-García,Alfonso Sinisterra,Rubén Dario Cortés,Maria Esperanza
author_sort	Gontijo,Sávio Morato de Lacerda
title	Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
title_short	Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
title_full	Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
title_fullStr	Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
title_full_unstemmed	Evaluation of antimicrobial activity and cell viability of Aloe vera sponges
title_sort	evaluation of antimicrobial activity and cell viability of aloe vera sponges
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100002
work_keys_str_mv	AT gontijosaviomoratodelacerda evaluationofantimicrobialactivityandcellviabilityofaloeverasponges AT gomesalinnedamasiamartins evaluationofantimicrobialactivityandcellviabilityofaloeverasponges AT galagarciaalfonso evaluationofantimicrobialactivityandcellviabilityofaloeverasponges AT sinisterrarubendario evaluationofantimicrobialactivityandcellviabilityofaloeverasponges AT cortesmariaesperanza evaluationofantimicrobialactivityandcellviabilityofaloeverasponges
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Evaluation of antimicrobial activity and cell viability of Aloe vera sponges

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