Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate
Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00)...
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Pontificia Universidad Católica de Valparaíso
2013
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oai:scielo:S0717-345820130002000052013-03-27Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrateBernal,MauricioTinoco,Lizeth KTorres,LuzMalagón-Romero,DionisioMontoya,Dolly anaerobic fermentation biofuel Clostridium spp glycerol, hydrogen mathematical model Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00). Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production). Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.2 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200005en10.2225/vol16-issue2-fulltext-4 |
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anaerobic fermentation biofuel Clostridium spp glycerol, hydrogen mathematical model |
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anaerobic fermentation biofuel Clostridium spp glycerol, hydrogen mathematical model Bernal,Mauricio Tinoco,Lizeth K Torres,Luz Malagón-Romero,Dionisio Montoya,Dolly Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
description |
Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00). Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production). Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible. |
author |
Bernal,Mauricio Tinoco,Lizeth K Torres,Luz Malagón-Romero,Dionisio Montoya,Dolly |
author_facet |
Bernal,Mauricio Tinoco,Lizeth K Torres,Luz Malagón-Romero,Dionisio Montoya,Dolly |
author_sort |
Bernal,Mauricio |
title |
Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
title_short |
Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
title_full |
Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
title_fullStr |
Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
title_full_unstemmed |
Evaluating Colombian Clostridium spp. Strains hydrogen production using glycerol as substrate |
title_sort |
evaluating colombian clostridium spp. strains hydrogen production using glycerol as substrate |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2013 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200005 |
work_keys_str_mv |
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