Effect of laundry detergent formulation on the performance of alkaline phytoproteases
Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful appl...
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Pontificia Universidad Católica de Valparaíso
2013
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oai:scielo:S0717-345820130003000032013-08-22Effect of laundry detergent formulation on the performance of alkaline phytoproteasesBarberis,SoniaQuiroga,EvelinaBarcia,CristinaLiggieri,Constanza asclepain araujiain detergents formulations alkaline plant proteases Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.3 20132013-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003en10.2225/vol16-issue3-fulltext-1 |
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Scielo Chile |
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Scielo Chile |
| language |
English |
| topic |
asclepain araujiain detergents formulations alkaline plant proteases |
| spellingShingle |
asclepain araujiain detergents formulations alkaline plant proteases Barberis,Sonia Quiroga,Evelina Barcia,Cristina Liggieri,Constanza Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| description |
Background: Proteases constitute the largest product segment in the global industrial enzymes market; they are used in food, pharmaceutical, leather, textile, wood and detergent industries. Alkaline proteases improve the cleaning efficiency of detergents and represent one of the most successful applications of modern industrial biotechnology. The aim of this work was to study the performance of two alkaline phytoproteases, araujiain (Araujia hortorum Fourn.) and asclepain (Asclepias curassavica L.), for their potential application as additive in laundry detergent formulations. Results: The effect of pure non-ionic and ionic surfactants on proteolytic activity of araujiain and asclepain was analyzed measuring the remaining activity after 1 hr of incubation of those enzymes in aqueous solutions of surfactants at different concentrations (0.1, 0.4 and 1% v/v) and temperatures (25, 40 and 60ºC). Besides, the compatibility of the enzymes with six commercial laundry detergents was also studied measuring the remaining proteolytic activity at 37ºC after 1 hr. Commercial detergent components influenced in different ways on araujiain and asclepain, in spite of the similar behaviour of the two enzymes in buffer. In commercial detergent solutions, araujiain expressed between 60% and 140% of its remaining proteolytic activity in buffer (pH 8.5) at 37ºC after 1 hr, while asclepain, was practically inactivate in most of them at the same conditions. Conclusions: Proteolytic extract of Araujia hortorum fulfilled all the requirements for its application as additive for laundry detergents: high stability in a broad temperature range (25-70ºC), high activity in alkaline pH (7.5-9.5) and very good compatibility with the commercial detergent additives. Nevertheless, in spite of its high stability and activity in buffer, the proteolytic extract of Asclepias curassavica did not show the same performance than araujiain. |
| author |
Barberis,Sonia Quiroga,Evelina Barcia,Cristina Liggieri,Constanza |
| author_facet |
Barberis,Sonia Quiroga,Evelina Barcia,Cristina Liggieri,Constanza |
| author_sort |
Barberis,Sonia |
| title |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| title_short |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| title_full |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| title_fullStr |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| title_full_unstemmed |
Effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| title_sort |
effect of laundry detergent formulation on the performance of alkaline phytoproteases |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300003 |
| work_keys_str_mv |
AT barberissonia effectoflaundrydetergentformulationontheperformanceofalkalinephytoproteases AT quirogaevelina effectoflaundrydetergentformulationontheperformanceofalkalinephytoproteases AT barciacristina effectoflaundrydetergentformulationontheperformanceofalkalinephytoproteases AT liggiericonstanza effectoflaundrydetergentformulationontheperformanceofalkalinephytoproteases |
| _version_ |
1718441871678636032 |