Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts

Enzymes are labile catalysts with reduced half-life time that can be however improved by immobilization and, furthermore, already inactivated catalyst can be recovered totally or partially, therefore allowing the large scale application of enzymes as process catalysts. In recent years a few studies...

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Autores principales:	Wilson,Lorena, Illanes,Andrés, Romero,Oscar
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	CLEAs enzyme immobilization enzyme reactivation glyoxyl agarose inactivation organic cosolvents unfolding - refolding
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300015
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spelling	oai:scielo:S0717-345820130003000152013-08-22Effect of inactivation and reactivation conditions on activity recovery of enzyme catalystsWilson,LorenaIllanes,AndrésRomero,Oscar CLEAs enzyme immobilization enzyme reactivation glyoxyl agarose inactivation organic cosolvents unfolding - refolding Enzymes are labile catalysts with reduced half-life time that can be however improved by immobilization and, furthermore, already inactivated catalyst can be recovered totally or partially, therefore allowing the large scale application of enzymes as process catalysts. In recent years a few studies about reactivation of enzyme catalysts have been published as a strategy to prolong the catalyst lifetime. Reported results are very good, making this strategy an interesting tool to be applied to industrial process. These studies have been focused in the evaluation of different variables that may have a positive impact both in the rate and level of activity recovery, being then critical variables for conducting the reactivation process at productive scale. The present work summarizes the studies done about reactivation strategies considering different variables: type of immobilization, enzyme-support interaction, level of catalyst inactivation prior to reactivation, temperature and presence of modulators.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.3 20132013-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300015en10.2225/vol16-issue3-fulltext-11
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CLEAs enzyme immobilization enzyme reactivation glyoxyl agarose inactivation organic cosolvents unfolding - refolding
spellingShingle	CLEAs enzyme immobilization enzyme reactivation glyoxyl agarose inactivation organic cosolvents unfolding - refolding Wilson,Lorena Illanes,Andrés Romero,Oscar Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
description	Enzymes are labile catalysts with reduced half-life time that can be however improved by immobilization and, furthermore, already inactivated catalyst can be recovered totally or partially, therefore allowing the large scale application of enzymes as process catalysts. In recent years a few studies about reactivation of enzyme catalysts have been published as a strategy to prolong the catalyst lifetime. Reported results are very good, making this strategy an interesting tool to be applied to industrial process. These studies have been focused in the evaluation of different variables that may have a positive impact both in the rate and level of activity recovery, being then critical variables for conducting the reactivation process at productive scale. The present work summarizes the studies done about reactivation strategies considering different variables: type of immobilization, enzyme-support interaction, level of catalyst inactivation prior to reactivation, temperature and presence of modulators.
author	Wilson,Lorena Illanes,Andrés Romero,Oscar
author_facet	Wilson,Lorena Illanes,Andrés Romero,Oscar
author_sort	Wilson,Lorena
title	Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
title_short	Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
title_full	Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
title_fullStr	Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
title_full_unstemmed	Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
title_sort	effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300015
work_keys_str_mv	AT wilsonlorena effectofinactivationandreactivationconditionsonactivityrecoveryofenzymecatalysts AT illanesandres effectofinactivationandreactivationconditionsonactivityrecoveryofenzymecatalysts AT romerooscar effectofinactivationandreactivationconditionsonactivityrecoveryofenzymecatalysts
_version_	1718441874307416064

Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts

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