Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses

Background: C-repeat binding factors (CBFs) are transcription factors that regulate the expression of a number of genes related to abiotic stresses. Few CBF genes have been cloned from other plants but no report in papaya. In present study, a full-length cDNA, designated as CpCBF2, was cloned from p...

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Autores principales:	Zhu,Xiaoyang, Li,Xueping, Chen,Weixin, Lu,Wangjin, Mao,Jia, Liu,Tongxin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	CpCBF2 gene expression papaya temperature stresses
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400001
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spelling	oai:scielo:S0717-345820130004000012013-08-22Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stressesZhu,XiaoyangLi,XuepingChen,WeixinLu,WangjinMao,JiaLiu,Tongxin CpCBF2 gene expression papaya temperature stresses Background: C-repeat binding factors (CBFs) are transcription factors that regulate the expression of a number of genes related to abiotic stresses. Few CBF genes have been cloned from other plants but no report in papaya. In present study, a full-length cDNA, designated as CpCBF2, was cloned from papaya using in silico cloning and 5’- rapid amplification cDNA ends (RACE). Sequence analysis was performed to understand the gene function. The expression pattern of CpCBF2 in papaya under low (7ºC) and high temperature (35ºC) stresses was examined using real-time quantitative polymerase chain reaction (RT-qPCR). Results: The full-length cDNA of CpCBF2 was 986-bp, with a 762-bp open reading frame (ORF) encoding a 254 amino acid polypeptide. CpCBF2 contained several major highly conserved regions including the CBF-family signature PKRRAGRKKFQETRHP and FADSAW in its amino acid sequence. Phylogenetic tree and three-dimensional structure analysis showed that CpCBF2 had a relatively close relationship with other plant CBFs. Gene expression analysis showed that high temperature stress had little effect on the expression of CpCBF2 but low temperature repressed CpCBF2 expression. Conclusion: The results showed that CpCBF2 may involve in different roles in temperature stress tolerance. This study provided a candidate gene potentially useful for fruit temperature stress tolerance, although its function still needs further confirmation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.4 20132013-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400001en10.2225/vol16-issue4-fulltext-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CpCBF2 gene expression papaya temperature stresses
spellingShingle	CpCBF2 gene expression papaya temperature stresses Zhu,Xiaoyang Li,Xueping Chen,Weixin Lu,Wangjin Mao,Jia Liu,Tongxin Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
description	Background: C-repeat binding factors (CBFs) are transcription factors that regulate the expression of a number of genes related to abiotic stresses. Few CBF genes have been cloned from other plants but no report in papaya. In present study, a full-length cDNA, designated as CpCBF2, was cloned from papaya using in silico cloning and 5’- rapid amplification cDNA ends (RACE). Sequence analysis was performed to understand the gene function. The expression pattern of CpCBF2 in papaya under low (7ºC) and high temperature (35ºC) stresses was examined using real-time quantitative polymerase chain reaction (RT-qPCR). Results: The full-length cDNA of CpCBF2 was 986-bp, with a 762-bp open reading frame (ORF) encoding a 254 amino acid polypeptide. CpCBF2 contained several major highly conserved regions including the CBF-family signature PKRRAGRKKFQETRHP and FADSAW in its amino acid sequence. Phylogenetic tree and three-dimensional structure analysis showed that CpCBF2 had a relatively close relationship with other plant CBFs. Gene expression analysis showed that high temperature stress had little effect on the expression of CpCBF2 but low temperature repressed CpCBF2 expression. Conclusion: The results showed that CpCBF2 may involve in different roles in temperature stress tolerance. This study provided a candidate gene potentially useful for fruit temperature stress tolerance, although its function still needs further confirmation.
author	Zhu,Xiaoyang Li,Xueping Chen,Weixin Lu,Wangjin Mao,Jia Liu,Tongxin
author_facet	Zhu,Xiaoyang Li,Xueping Chen,Weixin Lu,Wangjin Mao,Jia Liu,Tongxin
author_sort	Zhu,Xiaoyang
title	Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
title_short	Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
title_full	Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
title_fullStr	Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
title_full_unstemmed	Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses
title_sort	molecular cloning, characterization and expression analysis of cpcbf2 gene in harvested papaya fruit under temperature stresses
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400001
work_keys_str_mv	AT zhuxiaoyang molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses AT lixueping molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses AT chenweixin molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses AT luwangjin molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses AT maojia molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses AT liutongxin molecularcloningcharacterizationandexpressionanalysisofcpcbf2geneinharvestedpapayafruitundertemperaturestresses
_version_	1718441875030933504

Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses

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