3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila

Background: Interleukin-22 (IL-22) plays an important role in the regulation of immune responses. However, little is known about its function or structure in fish. Results: The IL-22 gene was first cloned from So-iny mullet (Liza haematocheila), one of commercially important fish species in China. T...

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Autores principales:	Qi,Zhitao, Shi,Rongwei, Li,Suyang, Zang,Xiayan, Wang,Zisheng
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	comparative modeling Interleukin-22 Liza haematocheila molecular dynamics
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400003
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spelling	oai:scielo:S0717-345820130004000032013-08-223-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheilaQi,ZhitaoShi,RongweiLi,SuyangZang,XiayanWang,Zisheng comparative modeling Interleukin-22 Liza haematocheila molecular dynamics Background: Interleukin-22 (IL-22) plays an important role in the regulation of immune responses. However, little is known about its function or structure in fish. Results: The IL-22 gene was first cloned from So-iny mullet (Liza haematocheila), one of commercially important fish species in China. Then, 3-D structure model of the mullet IL-22 was constructed by comparative modeling method using human IL-22 (1M4R) as template, and a 5 ns molecular dynamics (MD) was studied. The open reading frame (ORF) of mullet IL-22 cDNA was 555 bp, encoding 184 amino acids. The mullet IL-22 shared higher identities with the other fish IL-22 homologs and possessed a conserved IL-10 signature motif at its C-terminal. The mullet IL-22 model possessed six conserved helix structure. PROCHECK, SAVES and Molprobity server analysis confirmed that this model threaded well with human IL-22. Strikingly, analysis with CastP, cons-PPISP server suggested that the cysteines in mullet IL-22 might not be involved in the forming of disulfide bond for structural stabilization, but related to protein-protein interactions. Conclusions: The structure of IL-22 in So-iny mullet (Liza haematocheila) was constructed using comparative modeling method which provide more information for studying the function of fish IL-22.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.4 20132013-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400003en10.2225/vol16-issue4-fulltext-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	comparative modeling Interleukin-22 Liza haematocheila molecular dynamics
spellingShingle	comparative modeling Interleukin-22 Liza haematocheila molecular dynamics Qi,Zhitao Shi,Rongwei Li,Suyang Zang,Xiayan Wang,Zisheng 3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
description	Background: Interleukin-22 (IL-22) plays an important role in the regulation of immune responses. However, little is known about its function or structure in fish. Results: The IL-22 gene was first cloned from So-iny mullet (Liza haematocheila), one of commercially important fish species in China. Then, 3-D structure model of the mullet IL-22 was constructed by comparative modeling method using human IL-22 (1M4R) as template, and a 5 ns molecular dynamics (MD) was studied. The open reading frame (ORF) of mullet IL-22 cDNA was 555 bp, encoding 184 amino acids. The mullet IL-22 shared higher identities with the other fish IL-22 homologs and possessed a conserved IL-10 signature motif at its C-terminal. The mullet IL-22 model possessed six conserved helix structure. PROCHECK, SAVES and Molprobity server analysis confirmed that this model threaded well with human IL-22. Strikingly, analysis with CastP, cons-PPISP server suggested that the cysteines in mullet IL-22 might not be involved in the forming of disulfide bond for structural stabilization, but related to protein-protein interactions. Conclusions: The structure of IL-22 in So-iny mullet (Liza haematocheila) was constructed using comparative modeling method which provide more information for studying the function of fish IL-22.
author	Qi,Zhitao Shi,Rongwei Li,Suyang Zang,Xiayan Wang,Zisheng
author_facet	Qi,Zhitao Shi,Rongwei Li,Suyang Zang,Xiayan Wang,Zisheng
author_sort	Qi,Zhitao
title	3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
title_short	3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
title_full	3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
title_fullStr	3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
title_full_unstemmed	3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila
title_sort	3-d modeling and molecular dynamics simulation of interleukin-22 from the so-iny mullet, liza haematocheila
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400003
work_keys_str_mv	AT qizhitao 3dmodelingandmoleculardynamicssimulationofinterleukin22fromthesoinymulletlizahaematocheila AT shirongwei 3dmodelingandmoleculardynamicssimulationofinterleukin22fromthesoinymulletlizahaematocheila AT lisuyang 3dmodelingandmoleculardynamicssimulationofinterleukin22fromthesoinymulletlizahaematocheila AT zangxiayan 3dmodelingandmoleculardynamicssimulationofinterleukin22fromthesoinymulletlizahaematocheila AT wangzisheng 3dmodelingandmoleculardynamicssimulationofinterleukin22fromthesoinymulletlizahaematocheila
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3-D modeling and molecular dynamics simulation of interleukin-22 from the So-iny mullet, Liza haematocheila

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