Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation

Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lac...

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Autores principales:	Díaz,Rubén, Téllez-Téllez,Maura, Sánchez,Carmen, Bibbins-Martínez,Martha D, Díaz-Godínez,Gerardo, Soriano-Santos,Jorge
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	laccases Pleurotus ostreatus submerged fermentation zymogram
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400006
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spelling	oai:scielo:S0717-345820130004000062013-08-22Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentationDíaz,RubénTéllez-Téllez,MauraSánchez,CarmenBibbins-Martínez,Martha DDíaz-Godínez,GerardoSoriano-Santos,Jorge laccases Pleurotus ostreatus submerged fermentation zymogram Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures were also studied. Results: The highest laccases activity was obtained in cultures grown at initial pH of 4.5 and the lowest in cultures grown at initial pH of 8.5. Isoenzyme profiles were different in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all the cultures. Conclusions: The initial pH of the growing medium is an important factor for regulating the expression of laccase genes, having an effect on the activity and on the laccase isoenzymes number produced by P. ostreatus in SmF. This is the first report on the influence of different initial pH values of the growing medium on the laccases activity, laccase isoenzymes number and laccases expression profiles of P. ostreatus grown in submerged fermentation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.4 20132013-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400006en10.2225/vol16-issue4-fulltext-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	laccases Pleurotus ostreatus submerged fermentation zymogram
spellingShingle	laccases Pleurotus ostreatus submerged fermentation zymogram Díaz,Rubén Téllez-Téllez,Maura Sánchez,Carmen Bibbins-Martínez,Martha D Díaz-Godínez,Gerardo Soriano-Santos,Jorge Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
description	Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures were also studied. Results: The highest laccases activity was obtained in cultures grown at initial pH of 4.5 and the lowest in cultures grown at initial pH of 8.5. Isoenzyme profiles were different in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all the cultures. Conclusions: The initial pH of the growing medium is an important factor for regulating the expression of laccase genes, having an effect on the activity and on the laccase isoenzymes number produced by P. ostreatus in SmF. This is the first report on the influence of different initial pH values of the growing medium on the laccases activity, laccase isoenzymes number and laccases expression profiles of P. ostreatus grown in submerged fermentation.
author	Díaz,Rubén Téllez-Téllez,Maura Sánchez,Carmen Bibbins-Martínez,Martha D Díaz-Godínez,Gerardo Soriano-Santos,Jorge
author_facet	Díaz,Rubén Téllez-Téllez,Maura Sánchez,Carmen Bibbins-Martínez,Martha D Díaz-Godínez,Gerardo Soriano-Santos,Jorge
author_sort	Díaz,Rubén
title	Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
title_short	Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
title_full	Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
title_fullStr	Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
title_full_unstemmed	Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation
title_sort	influence of initial ph of the growing medium on the activity, production and genes expression profiles of laccase of pleurotus ostreatus in submerged fermentation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400006
work_keys_str_mv	AT diazruben influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation AT telleztellezmaura influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation AT sanchezcarmen influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation AT bibbinsmartinezmarthad influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation AT diazgodinezgerardo influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation AT sorianosantosjorge influenceofinitialphofthegrowingmediumontheactivityproductionandgenesexpressionprofilesoflaccaseofpleurotusostreatusinsubmergedfermentation
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Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation

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