Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils

Background: Cellulose can be converted to ethanol by simultaneous saccharification and fermentation (SSF). The difference between the optimal temperature of cellulase and microbial fermentation, however, has been identified as the critical problem with SSF. In this study, one fungal strain (AnsX1) w...

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Autores principales:	Wang,Nengfei, Zang,Jiaye, Ming,Kaili, Liu,Yu, Wu,Zuohao, Ding,Hui
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	Antarctic soil cellulose filter paper cellulase assay psychrotrophic fungi Verticillium sp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400010
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spelling	oai:scielo:S0717-345820130004000102013-08-22Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soilsWang,NengfeiZang,JiayeMing,KailiLiu,YuWu,ZuohaoDing,Hui Antarctic soil cellulose filter paper cellulase assay psychrotrophic fungi Verticillium sp Background: Cellulose can be converted to ethanol by simultaneous saccharification and fermentation (SSF). The difference between the optimal temperature of cellulase and microbial fermentation, however, has been identified as the critical problem with SSF. In this study, one fungal strain (AnsX1) with high cellulase activity at low temperature was isolated from Antarctic soils and identified as Verticillium sp. by morphological and molecular analyses. Results: The biochemical properties of crude AnsX1 cellulase samples were studied by filter paper cellulase assay. The maximum cellulase activity was achieved at low temperature in an acidic environment with addition of metal ions. Furthermore, AnsX1 cellulase demonstrated 54-63% enzymatic activity at ethanol concentrations of 5-10%. AnsX1 cellulase production was influenced by inoculum size, carbon and nitrogen sources, and elicitors. The optimal culture conditions for AnsX1 cellulase production were 5% inoculum, wheat bran as carbon source, (NH4)2SO4 as nitrogen source, and sorbitol added in the medium. Conclusions: Our present work has potential to enable the development of an economic and efficient cold-adapted cellulase system for bioconversion of lignocellulosic biomass into biofuels in future.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.4 20132013-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400010en10.2225/vol16-issue4-fulltext-12
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antarctic soil cellulose filter paper cellulase assay psychrotrophic fungi Verticillium sp
spellingShingle	Antarctic soil cellulose filter paper cellulase assay psychrotrophic fungi Verticillium sp Wang,Nengfei Zang,Jiaye Ming,Kaili Liu,Yu Wu,Zuohao Ding,Hui Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
description	Background: Cellulose can be converted to ethanol by simultaneous saccharification and fermentation (SSF). The difference between the optimal temperature of cellulase and microbial fermentation, however, has been identified as the critical problem with SSF. In this study, one fungal strain (AnsX1) with high cellulase activity at low temperature was isolated from Antarctic soils and identified as Verticillium sp. by morphological and molecular analyses. Results: The biochemical properties of crude AnsX1 cellulase samples were studied by filter paper cellulase assay. The maximum cellulase activity was achieved at low temperature in an acidic environment with addition of metal ions. Furthermore, AnsX1 cellulase demonstrated 54-63% enzymatic activity at ethanol concentrations of 5-10%. AnsX1 cellulase production was influenced by inoculum size, carbon and nitrogen sources, and elicitors. The optimal culture conditions for AnsX1 cellulase production were 5% inoculum, wheat bran as carbon source, (NH4)2SO4 as nitrogen source, and sorbitol added in the medium. Conclusions: Our present work has potential to enable the development of an economic and efficient cold-adapted cellulase system for bioconversion of lignocellulosic biomass into biofuels in future.
author	Wang,Nengfei Zang,Jiaye Ming,Kaili Liu,Yu Wu,Zuohao Ding,Hui
author_facet	Wang,Nengfei Zang,Jiaye Ming,Kaili Liu,Yu Wu,Zuohao Ding,Hui
author_sort	Wang,Nengfei
title	Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
title_short	Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
title_full	Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
title_fullStr	Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
title_full_unstemmed	Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils
title_sort	production of cold-adapted cellulase by verticillium sp. isolated from antarctic soils
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400010
work_keys_str_mv	AT wangnengfei productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils AT zangjiaye productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils AT mingkaili productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils AT liuyu productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils AT wuzuohao productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils AT dinghui productionofcoldadaptedcellulasebyverticilliumspisolatedfromantarcticsoils
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Production of cold-adapted cellulase by Verticillium sp. isolated from Antarctic soils

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