Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers

Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture...

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Autores principales:	Ravanfar,Seyed Ali, Aziz,Maheran Abdul, Shabanimofrad,Mahmoodreza, Samarfard,Samira
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	AtHSP101 gene Brassica oleracea ISSR transgenic broccoli
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500004
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spelling	oai:scielo:S0717-345820130005000042014-09-17Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markersRavanfar,Seyed AliAziz,Maheran AbdulShabanimofrad,MahmoodrezaSamarfard,Samira AtHSP101 gene Brassica oleracea ISSR transgenic broccoli Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgroTM) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that the transgenic plants could survive and performed normally, producing flower heads even at the highest tested temperature of 34ºC. Seven transgenic broccoli lines with different gene copy number of the AtHSP101 gene as well as the control plant were assessed for genetic diversity using inter simple sequence repeat (ISSR) markers. Conclusions: ISSR results showed polymorphism and phylogenetic relationship between the transgenic and non-transgenic (control) Brassica oleracea cv. Green Marvel.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.5 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500004en10.2225/vol16-issue5-fulltext-10
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AtHSP101 gene Brassica oleracea ISSR transgenic broccoli
spellingShingle	AtHSP101 gene Brassica oleracea ISSR transgenic broccoli Ravanfar,Seyed Ali Aziz,Maheran Abdul Shabanimofrad,Mahmoodreza Samarfard,Samira Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
description	Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgroTM) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that the transgenic plants could survive and performed normally, producing flower heads even at the highest tested temperature of 34ºC. Seven transgenic broccoli lines with different gene copy number of the AtHSP101 gene as well as the control plant were assessed for genetic diversity using inter simple sequence repeat (ISSR) markers. Conclusions: ISSR results showed polymorphism and phylogenetic relationship between the transgenic and non-transgenic (control) Brassica oleracea cv. Green Marvel.
author	Ravanfar,Seyed Ali Aziz,Maheran Abdul Shabanimofrad,Mahmoodreza Samarfard,Samira
author_facet	Ravanfar,Seyed Ali Aziz,Maheran Abdul Shabanimofrad,Mahmoodreza Samarfard,Samira
author_sort	Ravanfar,Seyed Ali
title	Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_short	Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_full	Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_fullStr	Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_full_unstemmed	Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_sort	greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (issr) markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500004
work_keys_str_mv	AT ravanfarseyedali greenhouseevaluationontheperformanceofheattoleranttransgenicbroccoliandgeneticdiversityanalysisusingintersimplesequencerepeatissrmarkers AT azizmaheranabdul greenhouseevaluationontheperformanceofheattoleranttransgenicbroccoliandgeneticdiversityanalysisusingintersimplesequencerepeatissrmarkers AT shabanimofradmahmoodreza greenhouseevaluationontheperformanceofheattoleranttransgenicbroccoliandgeneticdiversityanalysisusingintersimplesequencerepeatissrmarkers AT samarfardsamira greenhouseevaluationontheperformanceofheattoleranttransgenicbroccoliandgeneticdiversityanalysisusingintersimplesequencerepeatissrmarkers
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Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers

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