Studies on statistical optimization of sulforaphane production from broccoli seed

Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major...

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Autores principales:	Wu,Yuanfeng, Mao,Jianwei, Mei,Lehe, Liu,Shiwang
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	broccoli seed central composite design Plackett-Burman design production sulforaphane
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600001
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spelling	oai:scielo:S0717-345820130006000012014-01-16Studies on statistical optimization of sulforaphane production from broccoli seedWu,YuanfengMao,JianweiMei,LeheLiu,Shiwang broccoli seed central composite design Plackett-Burman design production sulforaphane Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major factors (hydrolysis time, water volume and ethyl acetate volume) were screened out through Plackett-Burman (PB) factorial design. The methods of steepest ascent combined with central composite design (CCD) were employed for optimization of the SF production process. The optimal extraction conditions for SF production were a hydrolysis time of 13 min, a hydrolysis volume/weight ratio of 2.9:1 (v/g) and an extraction volume/weight ratio of 17.5:1 (v/g). The maximum SF yield was 14.8 ± 0.1 mg/g, a value that was in perfect agreement with the actual experimental value (14.8 mg/g). Conclusions: These results suggested that PB design combined with CCD were proved effective in screening and optimization of the parameters of SF production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.6 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600001en10.2225/vol16-issue6-fulltext-16
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	broccoli seed central composite design Plackett-Burman design production sulforaphane
spellingShingle	broccoli seed central composite design Plackett-Burman design production sulforaphane Wu,Yuanfeng Mao,Jianwei Mei,Lehe Liu,Shiwang Studies on statistical optimization of sulforaphane production from broccoli seed
description	Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major factors (hydrolysis time, water volume and ethyl acetate volume) were screened out through Plackett-Burman (PB) factorial design. The methods of steepest ascent combined with central composite design (CCD) were employed for optimization of the SF production process. The optimal extraction conditions for SF production were a hydrolysis time of 13 min, a hydrolysis volume/weight ratio of 2.9:1 (v/g) and an extraction volume/weight ratio of 17.5:1 (v/g). The maximum SF yield was 14.8 ± 0.1 mg/g, a value that was in perfect agreement with the actual experimental value (14.8 mg/g). Conclusions: These results suggested that PB design combined with CCD were proved effective in screening and optimization of the parameters of SF production.
author	Wu,Yuanfeng Mao,Jianwei Mei,Lehe Liu,Shiwang
author_facet	Wu,Yuanfeng Mao,Jianwei Mei,Lehe Liu,Shiwang
author_sort	Wu,Yuanfeng
title	Studies on statistical optimization of sulforaphane production from broccoli seed
title_short	Studies on statistical optimization of sulforaphane production from broccoli seed
title_full	Studies on statistical optimization of sulforaphane production from broccoli seed
title_fullStr	Studies on statistical optimization of sulforaphane production from broccoli seed
title_full_unstemmed	Studies on statistical optimization of sulforaphane production from broccoli seed
title_sort	studies on statistical optimization of sulforaphane production from broccoli seed
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600001
work_keys_str_mv	AT wuyuanfeng studiesonstatisticaloptimizationofsulforaphaneproductionfrombroccoliseed AT maojianwei studiesonstatisticaloptimizationofsulforaphaneproductionfrombroccoliseed AT meilehe studiesonstatisticaloptimizationofsulforaphaneproductionfrombroccoliseed AT liushiwang studiesonstatisticaloptimizationofsulforaphaneproductionfrombroccoliseed
_version_	1718441881460801536

Studies on statistical optimization of sulforaphane production from broccoli seed

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