Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress

Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subt...

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Autores principales:	Zhong,Tianxiu, Zhu,Chen, Zeng,Huiming, Han,Liebao
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	bioinformatics analysis real time PCR suppression subtractive hybridization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004
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spelling	oai:scielo:S0717-345820130006000042014-01-16Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stressZhong,TianxiuZhu,ChenZeng,HuimingHan,Liebao bioinformatics analysis real time PCR suppression subtractive hybridization Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproduction. Leaves from plants subjected to drought stress provided the source of ‘Tester’ DNA, and leaves from plants grown under normal conditions provided the ‘Driver’ DNA for subtractive hybridization. Results: A total of 481 high quality ESTs were generated, which clustered into 390 unigenes. Of these unigenes, 132 grouped into 12 functional categories, suggesting that asexual reproduction is a complicated process involving a large number of genes. The expression characteristics of selected genes from the SSH library were determined by real-time PCR and were classified into five groups, suggesting that gene expression patterns during asexual reproduction are complex. Up-regulation of S-adenosylhomocysteine hydrolase suggested that a decrease in cytokinin levels promotes the initiation of plantlet formation. Many other genes, such as inorganic pyrophosphatase and glutamate decarboxylase, play important roles in gene regulation during asexual reproduction. Conclusion: Our results provide a framework and unified platform on which future research on asexual reproduction in K. daigremontiana can be based. This represents the first genome-wide study of asexual reproduction in K. daigremontiana.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.6 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004en10.2225/vol16-issue6-fulltext-14
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bioinformatics analysis real time PCR suppression subtractive hybridization
spellingShingle	bioinformatics analysis real time PCR suppression subtractive hybridization Zhong,Tianxiu Zhu,Chen Zeng,Huiming Han,Liebao Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
description	Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproduction. Leaves from plants subjected to drought stress provided the source of ‘Tester’ DNA, and leaves from plants grown under normal conditions provided the ‘Driver’ DNA for subtractive hybridization. Results: A total of 481 high quality ESTs were generated, which clustered into 390 unigenes. Of these unigenes, 132 grouped into 12 functional categories, suggesting that asexual reproduction is a complicated process involving a large number of genes. The expression characteristics of selected genes from the SSH library were determined by real-time PCR and were classified into five groups, suggesting that gene expression patterns during asexual reproduction are complex. Up-regulation of S-adenosylhomocysteine hydrolase suggested that a decrease in cytokinin levels promotes the initiation of plantlet formation. Many other genes, such as inorganic pyrophosphatase and glutamate decarboxylase, play important roles in gene regulation during asexual reproduction. Conclusion: Our results provide a framework and unified platform on which future research on asexual reproduction in K. daigremontiana can be based. This represents the first genome-wide study of asexual reproduction in K. daigremontiana.
author	Zhong,Tianxiu Zhu,Chen Zeng,Huiming Han,Liebao
author_facet	Zhong,Tianxiu Zhu,Chen Zeng,Huiming Han,Liebao
author_sort	Zhong,Tianxiu
title	Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
title_short	Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
title_full	Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
title_fullStr	Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
title_full_unstemmed	Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
title_sort	analysis of gene expression in kalanchoe daigremontiana leaves during plantlet formation under drought stress
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004
work_keys_str_mv	AT zhongtianxiu analysisofgeneexpressioninkalanchoedaigremontianaleavesduringplantletformationunderdroughtstress AT zhuchen analysisofgeneexpressioninkalanchoedaigremontianaleavesduringplantletformationunderdroughtstress AT zenghuiming analysisofgeneexpressioninkalanchoedaigremontianaleavesduringplantletformationunderdroughtstress AT hanliebao analysisofgeneexpressioninkalanchoedaigremontianaleavesduringplantletformationunderdroughtstress
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Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress

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