Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology
Background: β-Glucosidase is known as an effective catalyst for the hydrolysis of various glycosides and immobilization is one of the most efficient strategies to improve its activity recovery and properties. Results: Crosslinking-adsorption-crosslinking method was employed to immobilize &a...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2013
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600006 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582013000600006 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820130006000062014-01-16Optimal immobilization of β-glucosidase into chitosan beads using response surface methodologyZhou,YingWang,LufengWu,TingTang,XixuanPan,Siyi β-glucosidase characters chitosan beads immobilization response surface methodology Background: β-Glucosidase is known as an effective catalyst for the hydrolysis of various glycosides and immobilization is one of the most efficient strategies to improve its activity recovery and properties. Results: Crosslinking-adsorption-crosslinking method was employed to immobilize β-glucosidase into chitosan beads and response surface methodology (RSM) was used to optimize the immobilized conditions of the maximum activity recovery. Enzyme concentration and adsorption time were found to be significant influence factors, and the maximum activity recovery (50.75%) obtained from response surface methodology was in excellent agreement with experimental value (50.81%). Furthermore, various characteristics of immobilized β-glucosidase were evaluated. Compared to the free β-glucosidase, the immobilized enzyme exhibited broader pH and temperature ranges, enhanced thermal stability, better storage stability and reusability and higher accessibility of the substrate to the immobilized β-glucosidase. Conclusion: Response surface methodology (RSM) was proved to be much economical for optimum immobilization of β-glucosidase into chitosan beads.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.6 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600006en10.2225/vol16-issue6-fulltext-5 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
β-glucosidase characters chitosan beads immobilization response surface methodology |
| spellingShingle |
β-glucosidase characters chitosan beads immobilization response surface methodology Zhou,Ying Wang,Lufeng Wu,Ting Tang,Xixuan Pan,Siyi Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| description |
Background: β-Glucosidase is known as an effective catalyst for the hydrolysis of various glycosides and immobilization is one of the most efficient strategies to improve its activity recovery and properties. Results: Crosslinking-adsorption-crosslinking method was employed to immobilize β-glucosidase into chitosan beads and response surface methodology (RSM) was used to optimize the immobilized conditions of the maximum activity recovery. Enzyme concentration and adsorption time were found to be significant influence factors, and the maximum activity recovery (50.75%) obtained from response surface methodology was in excellent agreement with experimental value (50.81%). Furthermore, various characteristics of immobilized β-glucosidase were evaluated. Compared to the free β-glucosidase, the immobilized enzyme exhibited broader pH and temperature ranges, enhanced thermal stability, better storage stability and reusability and higher accessibility of the substrate to the immobilized β-glucosidase. Conclusion: Response surface methodology (RSM) was proved to be much economical for optimum immobilization of β-glucosidase into chitosan beads. |
| author |
Zhou,Ying Wang,Lufeng Wu,Ting Tang,Xixuan Pan,Siyi |
| author_facet |
Zhou,Ying Wang,Lufeng Wu,Ting Tang,Xixuan Pan,Siyi |
| author_sort |
Zhou,Ying |
| title |
Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| title_short |
Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| title_full |
Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| title_fullStr |
Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| title_full_unstemmed |
Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| title_sort |
optimal immobilization of β-glucosidase into chitosan beads using response surface methodology |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600006 |
| work_keys_str_mv |
AT zhouying optimalimmobilizationof946glucosidaseintochitosanbeadsusingresponsesurfacemethodology AT wanglufeng optimalimmobilizationof946glucosidaseintochitosanbeadsusingresponsesurfacemethodology AT wuting optimalimmobilizationof946glucosidaseintochitosanbeadsusingresponsesurfacemethodology AT tangxixuan optimalimmobilizationof946glucosidaseintochitosanbeadsusingresponsesurfacemethodology AT pansiyi optimalimmobilizationof946glucosidaseintochitosanbeadsusingresponsesurfacemethodology |
| _version_ |
1718441882677149696 |