Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic...
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Pontificia Universidad Católica de Valparaíso
2013
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oai:scielo:S0717-345820130006000122014-01-16Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thalianaLi,ZhongaiDu,YaqiongWang,Zicheng Arabidopsis thaliana cryopreservation real-time PCR transformation efficiency Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and our observation was not in agreement with earlier reports. Transgenic Arabidopsis lines over-expressing ATOST1 gene were used for the real-time PCR analysis, and the result indicated that the expression of the ATOST1 gene was up-regulated about 2.4-fold in the transgenic seedlings tissues retrieved from cryopreservation than those non-cryopreserved counterparts. Conclusions： Cryopreservation could improve the expression of exogenous gene, however, could not promote the genetic transformation obviously.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.6 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600012en10.2225/vol16-issue6-fulltext-12 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Arabidopsis thaliana cryopreservation real-time PCR transformation efficiency |
| spellingShingle |
Arabidopsis thaliana cryopreservation real-time PCR transformation efficiency Li,Zhongai Du,Yaqiong Wang,Zicheng Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| description |
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and our observation was not in agreement with earlier reports. Transgenic Arabidopsis lines over-expressing ATOST1 gene were used for the real-time PCR analysis, and the result indicated that the expression of the ATOST1 gene was up-regulated about 2.4-fold in the transgenic seedlings tissues retrieved from cryopreservation than those non-cryopreserved counterparts. Conclusions： Cryopreservation could improve the expression of exogenous gene, however, could not promote the genetic transformation obviously. |
| author |
Li,Zhongai Du,Yaqiong Wang,Zicheng |
| author_facet |
Li,Zhongai Du,Yaqiong Wang,Zicheng |
| author_sort |
Li,Zhongai |
| title |
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| title_short |
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| title_full |
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| title_fullStr |
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| title_full_unstemmed |
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana |
| title_sort |
effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of arabidopsis thaliana |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600012 |
| work_keys_str_mv |
AT lizhongai effectofcryopreservationontheefficiencyofexogenousgenegenetictransformationandexpressionlevelofarabidopsisthaliana AT duyaqiong effectofcryopreservationontheefficiencyofexogenousgenegenetictransformationandexpressionlevelofarabidopsisthaliana AT wangzicheng effectofcryopreservationontheefficiencyofexogenousgenegenetictransformationandexpressionlevelofarabidopsisthaliana |
| _version_ |
1718441884425125888 |