New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry

DNA topoisomerases are essential enzymes that control the topological state of DNA replication during mitosis. These enzymes are classified based on their mechanisms and physical properties. During mitosis, superhelical DNA must be unwound or relaxed by DNA topoisomerases prior to a decoding step by...

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Autores principales:	Chan,Mooi Kwai, Fadzil,Nur Adila, Chew,Ai Lan, Khoo,Boon Yin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2013
Materias:	DNA topoisomerase medical laboratory application pharmaceutical industry recombinant DNA technology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600018
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spelling	oai:scielo:S0717-345820130006000182014-01-16New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industryChan,Mooi KwaiFadzil,Nur AdilaChew,Ai LanKhoo,Boon Yin DNA topoisomerase medical laboratory application pharmaceutical industry recombinant DNA technology DNA topoisomerases are essential enzymes that control the topological state of DNA replication during mitosis. These enzymes are classified based on their mechanisms and physical properties. During mitosis, superhelical DNA must be unwound or relaxed by DNA topoisomerases prior to a decoding step by DNA processing enzymes, such as DNA polymerase and RNA polymerase. By blocking the reaction of resealing the breaks in the DNA ultimately can result in cellular death. Compounds that inhibit the catalytic function of these enzymes can serve as potential anticancer agents. DNA topoisomerases are found in nature and used as high quality and well-validated targets for the screening of potential anticancer agents. Our current work focuses on determining potential anticancer agents from natural resources using DNA topoisomerases as the screening targets. Large scale production of these enzymes using recombinant DNA technology in our academic laboratory is utilised to avoid dependence on expensive commercially available enzymes. The in-house produced enzymes can also be used to enhance our research in the field of molecular medicine by providing an enzyme source that can be used to screen potential anticancer agents, and for other newly developed diagnostic and medical research projects in the near future as well as a step in moving our efforts into the industrial sector.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.6 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600018en10.2225/vol16-issue6-fulltext-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA topoisomerase medical laboratory application pharmaceutical industry recombinant DNA technology
spellingShingle	DNA topoisomerase medical laboratory application pharmaceutical industry recombinant DNA technology Chan,Mooi Kwai Fadzil,Nur Adila Chew,Ai Lan Khoo,Boon Yin New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
description	DNA topoisomerases are essential enzymes that control the topological state of DNA replication during mitosis. These enzymes are classified based on their mechanisms and physical properties. During mitosis, superhelical DNA must be unwound or relaxed by DNA topoisomerases prior to a decoding step by DNA processing enzymes, such as DNA polymerase and RNA polymerase. By blocking the reaction of resealing the breaks in the DNA ultimately can result in cellular death. Compounds that inhibit the catalytic function of these enzymes can serve as potential anticancer agents. DNA topoisomerases are found in nature and used as high quality and well-validated targets for the screening of potential anticancer agents. Our current work focuses on determining potential anticancer agents from natural resources using DNA topoisomerases as the screening targets. Large scale production of these enzymes using recombinant DNA technology in our academic laboratory is utilised to avoid dependence on expensive commercially available enzymes. The in-house produced enzymes can also be used to enhance our research in the field of molecular medicine by providing an enzyme source that can be used to screen potential anticancer agents, and for other newly developed diagnostic and medical research projects in the near future as well as a step in moving our efforts into the industrial sector.
author	Chan,Mooi Kwai Fadzil,Nur Adila Chew,Ai Lan Khoo,Boon Yin
author_facet	Chan,Mooi Kwai Fadzil,Nur Adila Chew,Ai Lan Khoo,Boon Yin
author_sort	Chan,Mooi Kwai
title	New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
title_short	New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
title_full	New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
title_fullStr	New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
title_full_unstemmed	New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry
title_sort	new molecular biologist perspective and insight: dna topoisomerases production by recombinant dna technology for medical laboratory application and pharmaceutical industry
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600018
work_keys_str_mv	AT chanmooikwai newmolecularbiologistperspectiveandinsightdnatopoisomerasesproductionbyrecombinantdnatechnologyformedicallaboratoryapplicationandpharmaceuticalindustry AT fadzilnuradila newmolecularbiologistperspectiveandinsightdnatopoisomerasesproductionbyrecombinantdnatechnologyformedicallaboratoryapplicationandpharmaceuticalindustry AT chewailan newmolecularbiologistperspectiveandinsightdnatopoisomerasesproductionbyrecombinantdnatechnologyformedicallaboratoryapplicationandpharmaceuticalindustry AT khooboonyin newmolecularbiologistperspectiveandinsightdnatopoisomerasesproductionbyrecombinantdnatechnologyformedicallaboratoryapplicationandpharmaceuticalindustry
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New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry

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