Production and characterization of algae extract from Chlamydomonas reinhardtii

Background: Algae offer many advantages as biofuel sources including: high growth rates, high lipid content, the ability to grow on non-agricultural land, and the genetic versatility to improve strains rapidly and produce co-products. Research is ongoing to make algae biofuels a more financially att...

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Autores principales:	Kightlinger,Weston, Chen,Kai, Pourmir,Azadeh, Crunkleton,Daniel W, Price,Geoffrey L, Johannes,Tyler W
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Algae extract Algae Chlamydomonas reinhardtii Microalgae Culture media
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100003
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spelling	oai:scielo:S0717-345820140001000032014-04-07Production and characterization of algae extract from Chlamydomonas reinhardtiiKightlinger,WestonChen,KaiPourmir,AzadehCrunkleton,Daniel WPrice,Geoffrey LJohannes,Tyler W Algae extract Algae Chlamydomonas reinhardtii Microalgae Culture media Background: Algae offer many advantages as biofuel sources including: high growth rates, high lipid content, the ability to grow on non-agricultural land, and the genetic versatility to improve strains rapidly and produce co-products. Research is ongoing to make algae biofuels a more financially attractive energy option; however, it is becoming evident that the economic viability of algae-based fuels may hinge upon high-value co-products. This work evaluated the feasibility of using a co-product, algae extract, as a nutrient source in cell culture media. Results: Algae extract prepared from autolysed Chlamydomonas reinhardtii was found to contain 3.0% protein, 9.2% total carbohydrate, and 3.9% free α-amino acid which is similar to the nutrient content of commercially available yeast extract. The effects of algae extract on the growth and metabolism of laboratory strains of Escherichia coli and Saccharomyces cerevisiae were tested by substituting algae extract for yeast extract in LB and YPAD growth media recipes. Complex laboratory media supplemented with algae extract instead of yeast extract showed markedly improved effects on the growth and metabolism of common laboratory microorganisms in all cases except ethanol production rates in yeast. Conclusions: This study showed that algae extract derived from C. reinhardtii is similar, if not superior, to commercially available yeast extract in nutrient content and effects on the growth and metabolism of E. coli and S. cerevisiae. Bacto™ yeast extract is valued at USD $0.15-0.35 per gram, if algae extract was sold at similar prices, it would serve as a high-value co-product in algae-based fuel processes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.1 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100003en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Algae extract Algae Chlamydomonas reinhardtii Microalgae Culture media
spellingShingle	Algae extract Algae Chlamydomonas reinhardtii Microalgae Culture media Kightlinger,Weston Chen,Kai Pourmir,Azadeh Crunkleton,Daniel W Price,Geoffrey L Johannes,Tyler W Production and characterization of algae extract from Chlamydomonas reinhardtii
description	Background: Algae offer many advantages as biofuel sources including: high growth rates, high lipid content, the ability to grow on non-agricultural land, and the genetic versatility to improve strains rapidly and produce co-products. Research is ongoing to make algae biofuels a more financially attractive energy option; however, it is becoming evident that the economic viability of algae-based fuels may hinge upon high-value co-products. This work evaluated the feasibility of using a co-product, algae extract, as a nutrient source in cell culture media. Results: Algae extract prepared from autolysed Chlamydomonas reinhardtii was found to contain 3.0% protein, 9.2% total carbohydrate, and 3.9% free α-amino acid which is similar to the nutrient content of commercially available yeast extract. The effects of algae extract on the growth and metabolism of laboratory strains of Escherichia coli and Saccharomyces cerevisiae were tested by substituting algae extract for yeast extract in LB and YPAD growth media recipes. Complex laboratory media supplemented with algae extract instead of yeast extract showed markedly improved effects on the growth and metabolism of common laboratory microorganisms in all cases except ethanol production rates in yeast. Conclusions: This study showed that algae extract derived from C. reinhardtii is similar, if not superior, to commercially available yeast extract in nutrient content and effects on the growth and metabolism of E. coli and S. cerevisiae. Bacto™ yeast extract is valued at USD $0.15-0.35 per gram, if algae extract was sold at similar prices, it would serve as a high-value co-product in algae-based fuel processes.
author	Kightlinger,Weston Chen,Kai Pourmir,Azadeh Crunkleton,Daniel W Price,Geoffrey L Johannes,Tyler W
author_facet	Kightlinger,Weston Chen,Kai Pourmir,Azadeh Crunkleton,Daniel W Price,Geoffrey L Johannes,Tyler W
author_sort	Kightlinger,Weston
title	Production and characterization of algae extract from Chlamydomonas reinhardtii
title_short	Production and characterization of algae extract from Chlamydomonas reinhardtii
title_full	Production and characterization of algae extract from Chlamydomonas reinhardtii
title_fullStr	Production and characterization of algae extract from Chlamydomonas reinhardtii
title_full_unstemmed	Production and characterization of algae extract from Chlamydomonas reinhardtii
title_sort	production and characterization of algae extract from chlamydomonas reinhardtii
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100003
work_keys_str_mv	AT kightlingerweston productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii AT chenkai productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii AT pourmirazadeh productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii AT crunkletondanielw productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii AT pricegeoffreyl productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii AT johannestylerw productionandcharacterizationofalgaeextractfromchlamydomonasreinhardtii
_version_	1718441886628184064

Production and characterization of algae extract from Chlamydomonas reinhardtii

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