The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer

Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li,Li, Zhong,Cai-hong, Bian,Yin-bing
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Gremplasm resource Genetic polymorphism Strain identification Ribosomal RNA genes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100005
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582014000100005
record_format	dspace
spelling	oai:scielo:S0717-345820140001000052014-04-07The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacerLi,LiZhong,Cai-hongBian,Yin-bing Gremplasm resource Genetic polymorphism Strain identification Ribosomal RNA genes Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibility to environmental influences. For this purpose, the molecular diversity of 32 A. auricula-judae commercial cultivars in China was analyzed by using the nuclear ribosomal DNA intergenic spacer. Results: The complete nuclear rDNA gene complex of A. auricula-judae isolate is 11,210 bp long, and contains the 18S, 5.8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four more effective primer combinations for the IGS region of A. auricula-judae were designed. Nucleotide sequence variation in the IGS among 32 A. auricula-judae commercial cultivars in China sorted into three strongly supported clades, which is correlated with geographical regions. Most strains originated from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains. Conclusion: The grouping information obtained in the present work provides significant information for further genetic improvement in A. auricula-judae, and suggested that the IGS region can be used as an excellent tool for identification of genetic variation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.1 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gremplasm resource Genetic polymorphism Strain identification Ribosomal RNA genes
spellingShingle	Gremplasm resource Genetic polymorphism Strain identification Ribosomal RNA genes Li,Li Zhong,Cai-hong Bian,Yin-bing The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
description	Background: For the crossbreeding of Auricularia auricula-judae, selecting the appropriated parents in hybridization is very important. However, the classification and diversity analysis of A. auricula-judae has been equivocal, due to the similarity of the fruiting body morphology and its susceptibility to environmental influences. For this purpose, the molecular diversity of 32 A. auricula-judae commercial cultivars in China was analyzed by using the nuclear ribosomal DNA intergenic spacer. Results: The complete nuclear rDNA gene complex of A. auricula-judae isolate is 11,210 bp long, and contains the 18S, 5.8S, and 28S rRNA gene as well as the ITS and IGS regions. Based on the sequence data, four more effective primer combinations for the IGS region of A. auricula-judae were designed. Nucleotide sequence variation in the IGS among 32 A. auricula-judae commercial cultivars in China sorted into three strongly supported clades, which is correlated with geographical regions. Most strains originated from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains. Conclusion: The grouping information obtained in the present work provides significant information for further genetic improvement in A. auricula-judae, and suggested that the IGS region can be used as an excellent tool for identification of genetic variation.
author	Li,Li Zhong,Cai-hong Bian,Yin-bing
author_facet	Li,Li Zhong,Cai-hong Bian,Yin-bing
author_sort	Li,Li
title	The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
title_short	The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
title_full	The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
title_fullStr	The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
title_full_unstemmed	The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer
title_sort	molecular diversity analysis of auricularia auricula-judae in china by nuclear ribosomal dna intergenic spacer
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100005
work_keys_str_mv	AT lili themoleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer AT zhongcaihong themoleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer AT bianyinbing themoleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer AT lili moleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer AT zhongcaihong moleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer AT bianyinbing moleculardiversityanalysisofauriculariaauriculajudaeinchinabynuclearribosomaldnaintergenicspacer
_version_	1718441887103188992

The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer

Ejemplares similares